In-vitro experimental evaluation of skin-to-surface recovery of four bacterial species by antibacterial and non-antibacterial medical examination gloves

Antimicrobial Resistance and Infection Control

Johannes Leitgeb²  Rupert Schuster²  Aik-Hwee Eng¹  Bit-New Yee¹  Yee-Peng Teh¹  Verena Dosch³  Ojan Assadian³ 

[1] Ansell Healthcare Products LLC, S&T Innovation Centre, 40000 Shah Alam Selangor, Malaysia

[2] Department for Trauma Surgery, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria

[3] Department for Hospital Hygiene, Medical University of Vienna, Waehringer Guertel 18-20, A-1090 Vienna, Austria

关键词: Cross-contamination;    PHMB;    Polyhexanide;    Antimicrobial glove;    Surface contamination;    Examination glove;   

Others  :  790743
DOI  :  10.1186/2047-2994-2-27

 received in 2013-03-13, accepted in 2013-10-04,  发布年份 2013

【 摘 要 】

Background

The number of bacteria recovered from a stainless steel coupon after touching a pigskin substrate with an examination glove coated on its outside with polyhexanide (PHMB), as compared to the number of bacteria recovered in the same manner with non-coated control gloves was evaluated.

Methods

Suspensions containing 1 × 10⁹ colony-forming units of 4 clinically relevant bacterial species (Enterococcus faecium ATCC #51559; Escherichia coli ATCC #25922; Klebsiella pneumoniae ATCC #4352; and Staphylococcus aureus ATCC #33591) were used to contaminate Gamma-irradiated pigskin substrates. Bacterial recoveries from the pigskin substrate, stainless steel coupons, and each glove swatch were performed. A difference in the bacterial recovery from the stainless steel coupons after touching with coated and uncoated control gloves was measured.

Results

For E. faecium, the coated glove showed a reduction of 4.63 log₁₀ cfu recovery, when compared to control gloves. For E. coli, the coated glove showed 5.48 log₁₀ cfu, for K. pneumoniae 5.03 log₁₀ cfu, and for S. aureus 5.72 log₁₀ cfu recovery, when compared to the non-coated control glove.

Conclusion

An in-vitro experiment designed to mimic cross-contamination of clinically relevant bacteria in a simulated healthcare setting following glove contact with a contaminated biological surface and cross-transfer to a stainless steel surface has demonstrated that an examination glove coated on its outside surface with PHMB was able to reduce bacterial recovery from a contaminated surface by > 4 log₁₀ cfu, compared to a control non-coated examination glove. These elaborated results may encourage further clinical investigation on the clinical impact of an antibacterial examination glove.

【 授权许可】

   
2013 Leitgeb et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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