Rotavirus and Norovirus infections among acute gastroenteritis children in Morocco

BMC Infectious Diseases

Research Article

Khalid Sadki¹  Saaid Amzazi¹  Maria El Qazoui²  Nezha El Omari²  Hicham Oumzil²  Mohamed Benhafid²  Larbi Baassi³  Rajae El Aouad⁴ 

[1] Department of Biology, Faculty of Sciences, Laboratory of Biochemistry and Immunology, B.P. 1014 RP, 4 Avenue Ibn Batouta, Rabat, Morocco;Immunology–Virology Department, National Institute of Hygiene, Ministry of Health, 27 Avenue Ibn Batouta, Rabat, Morocco;Office of the Laboratories of the National Institute of Hygiene, Ministry of Health, 27 Avenue Ibn Batouta, Rabat, Morocco;UPR Immunology-School of Medicine and Pharmacy, Mohamed V University, Avenue Mohamed Belarbi El Alaoui Souissi, Rabat, Morocco;

关键词: Norovirus;    Rotavirus;    Acute gastroenteritis;    Morocco;   

DOI  :  10.1186/1471-2334-14-300

 received in 2014-04-16, accepted in 2014-05-15,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundAcute gastroenteritis is a serious cause of child mortality and morbidity in resource-limited countries. A viral etiology is most common, and rotavirus and norovirus are reported to be the leading causative agents. There are still few epidemiological data on the simultaneous occurrence of these viruses in Morocco. The aim of this study was to provide useful epidemiological data on the gastroenteritis associated with rotavirus and norovirus among children aged less than 5 years.MethodsFrom January to December 2011, 335 samples were tested for rotavirus and norovirus using enzyme-linked immunosorbent assay, reverse-transcription-polymerase chain reaction (RT-multiplex PCR) and real-time RT-PCR. Partial sequences of the norovirus were phylogenetically analyzed to determine the genotype.ResultsThe overall rates of rotavirus and norovirus infections were 26.6% and 16.1%, respectively. Mixed viral infections were detected in 9 of 335 stool specimens (2.7%).The most common genotype combination in the rotavirus strains was G1[P8] (51.7%), followed by G2[P4] (10.1%), G2[P8] (4.5%), G9[P8] (3.4%), G4[P8] (3.4%), and G1[P6] (2.3%). Among patients positive for norovirus, 42 (77.8%) tested positive for GII and 12 (22.2%) for GI. Thirty-three (78.6%) of the norovirus GII-positive cases were successfully characterized. Genotype GII.4 was the most prevalent (n = 27; 81.8%), followed by GII.3 (n = 2; 6.1%), GII.13 (n = 2; 6.1%), GII.16 (n = 1; 3%), and GII.17 (n = 1; 3%).ConclusionThis study suggests that in Morocco, norovirus is the most frequent cause of acute gastroenteritis after rotavirus, but further enteric viruses need to be integrated in the surveillance system so that a conclusion could be drawn.

【 授权许可】

CC BY   
© El Qazoui et al.; licensee BioMed Central Ltd. 2014