Rotavirus and Norovirus infections among acute gastroenteritis children in Morocco

BMC Infectious Diseases

Rajae El Aouad³  Mohamed Benhafid²  Saaid Amzazi⁴  Khalid Sadki⁴  Nezha El Omari²  Larbi Baassi¹  Hicham Oumzil²  Maria El Qazoui² 

[1] Office of the Laboratories of the National Institute of Hygiene, Ministry of Health, 27 Avenue Ibn Batouta, Rabat, Morocco;Immunology–Virology Department, National Institute of Hygiene, Ministry of Health, 27 Avenue Ibn Batouta, Rabat, Morocco;UPR Immunology-School of Medicine and Pharmacy, Mohamed V University, Avenue Mohamed Belarbi El Alaoui Souissi, Rabat, Morocco;Laboratory of Biochemistry and Immunology, Department of Biology, Faculty of Sciences, 4 Avenue Ibn Batouta, B.P. 1014 RP, Rabat, Morocco

关键词: Morocco;    Acute gastroenteritis;    Rotavirus;    Norovirus;   

Others  :  1127683
DOI  :  10.1186/1471-2334-14-300

 received in 2014-04-16, accepted in 2014-05-15,  发布年份 2014

【 摘 要 】

Background

Acute gastroenteritis is a serious cause of child mortality and morbidity in resource-limited countries. A viral etiology is most common, and rotavirus and norovirus are reported to be the leading causative agents. There are still few epidemiological data on the simultaneous occurrence of these viruses in Morocco. The aim of this study was to provide useful epidemiological data on the gastroenteritis associated with rotavirus and norovirus among children aged less than 5 years.

Methods

From January to December 2011, 335 samples were tested for rotavirus and norovirus using enzyme-linked immunosorbent assay, reverse-transcription-polymerase chain reaction (RT-multiplex PCR) and real-time RT-PCR. Partial sequences of the norovirus were phylogenetically analyzed to determine the genotype.

Results

The overall rates of rotavirus and norovirus infections were 26.6% and 16.1%, respectively. Mixed viral infections were detected in 9 of 335 stool specimens (2.7%).

The most common genotype combination in the rotavirus strains was G1[P8] (51.7%), followed by G2[P4] (10.1%), G2[P8] (4.5%), G9[P8] (3.4%), G4[P8] (3.4%), and G1[P6] (2.3%). Among patients positive for norovirus, 42 (77.8%) tested positive for GII and 12 (22.2%) for GI. Thirty-three (78.6%) of the norovirus GII-positive cases were successfully characterized. Genotype GII.4 was the most prevalent (n = 27; 81.8%), followed by GII.3 (n = 2; 6.1%), GII.13 (n = 2; 6.1%), GII.16 (n = 1; 3%), and GII.17 (n = 1; 3%).

Conclusion

This study suggests that in Morocco, norovirus is the most frequent cause of acute gastroenteritis after rotavirus, but further enteric viruses need to be integrated in the surveillance system so that a conclusion could be drawn.

【 授权许可】

   
2014 El Qazoui et al.; licensee BioMed Central Ltd.

【 图 表 】

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