Anti-inflammatory effect of Prunus yedoensis through inhibition of nuclear factor-κB in macrophages

BMC Complementary and Alternative Medicine

Research Article

Mi-Hwa Lee¹  Kyungjin Lee¹  Gabsik Yang¹  Inhye Ham¹  Juyeong Lee¹  Ho-Young Choi²  Ji-Whan Eom³ 

[1] Department of Herbology, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul, Republic of Korea;Department of Herbology, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul, Republic of Korea;College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul, Republic of Korea;Department of Physiology, College of Oriental Medicine, Sangji University, 220-702, Wonju-si, Gangwon-do, Republic of Korea;

关键词: Prunus yedoensis;    Inducible nitric oxide synthase;    Cyclooxygenase-2;    Nuclear factor-κB;   

DOI  :  10.1186/1472-6882-13-92

 received in 2012-10-10, accepted in 2013-04-19,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundPrunus yedoensis (PY) is a traditional anti-allergy and anti-inflammatory herb medicine used in South Korea. However, until date, little is known regarding its mechanism of action.MethodsIn order to elucidate the mechanism of anti-inflammatory effect of PY, the constituents of PY were analysed by high performance liquid chromatography (HPLC), and nitric oxide (NO) and prostaglandin E2 (PGE2) production were measured enzyme-linked immuno sorbent assay (ELISA). The expression levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear factor-κB (NF-κB) were also measured by western blotting in lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells treated with PY.ResultsThe results indicate that (50, 100 μg/mL) methanol and ethyl acetate fractionation extracts of PY not only inhibited LPS-mediated NO production and iNOS expression, but also decreased LPS-induced PGE2 production and COX-2 expression. The anti-inflammatory effects of PY were also due to the attenuation of nuclear translocation of NF-κB, as evaluated by the use of anti-p50 on nuclear fractions. LPS-induced nuclear translocation of NF-κB decreased significantly by the methanol extract and ethyl acetate fraction of PY. High performance liquid chromatography (HPLC) analyses revealed that methanol extract and ethyl acetate fraction have similar patterns of retention time and peaks.ConclusionOur results demonstrate that methanol extracts and the ethyl acetate fraction of PY have anti-inflammatory properties, thus emphasizing the potential of PY as a natural health product.

【 授权许可】

Unknown   
© Lee et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.