Anti-inflammatory effect of Prunus yedoensis through inhibition of nuclear factor-κB in macrophages

BMC Complementary and Alternative Medicine

Ho-Young Choi²  Inhye Ham¹  Ji-Whan Eom³  Mi-Hwa Lee¹  Kyungjin Lee¹  Gabsik Yang¹  Juyeong Lee¹ 

[1] Department of Herbology, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul, Republic of Korea;College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul, Republic of Korea;Department of Physiology, College of Oriental Medicine, Sangji University, Wonju-si Gangwon-do 220-702, Republic of Korea

关键词: Nuclear factor-κB;    Cyclooxygenase-2;    Inducible nitric oxide synthase;    Prunus yedoensis;   

Others  :  1230059
DOI  :  10.1186/1472-6882-13-92

 received in 2012-10-10, accepted in 2013-04-19,  发布年份 2013

【 摘 要 】

Background

Prunus yedoensis (PY) is a traditional anti-allergy and anti-inflammatory herb medicine used in South Korea. However, until date, little is known regarding its mechanism of action.

Methods

In order to elucidate the mechanism of anti-inflammatory effect of PY, the constituents of PY were analysed by high performance liquid chromatography (HPLC), and nitric oxide (NO) and prostaglandin E₂ (PGE₂) production were measured enzyme-linked immuno sorbent assay (ELISA). The expression levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear factor-κB (NF-κB) were also measured by western blotting in lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells treated with PY.

Results

The results indicate that (50, 100 μg/mL) methanol and ethyl acetate fractionation extracts of PY not only inhibited LPS-mediated NO production and iNOS expression, but also decreased LPS-induced PGE₂ production and COX-2 expression. The anti-inflammatory effects of PY were also due to the attenuation of nuclear translocation of NF-κB, as evaluated by the use of anti-p50 on nuclear fractions. LPS-induced nuclear translocation of NF-κB decreased significantly by the methanol extract and ethyl acetate fraction of PY. High performance liquid chromatography (HPLC) analyses revealed that methanol extract and ethyl acetate fraction have similar patterns of retention time and peaks.

Conclusion

Our results demonstrate that methanol extracts and the ethyl acetate fraction of PY have anti-inflammatory properties, thus emphasizing the potential of PY as a natural health product.

【 授权许可】

   
2013 Lee et al.; licensee BioMed Central Ltd.

【 图 表 】

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