期刊论文详细信息
BMC Complementary and Alternative Medicine
Cranberry proanthocyanidins have anti-biofilm properties against Pseudomonas aeruginosa
Research Article
Weidong Zhou1  Robert K Ulrey2  Stephanie M Barksdale3  Monique L van Hoek4 
[1] Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, Virginia, USA;Department of Biology, George Mason University, Manassas, Virginia, USA;School of Systems Biology, George Mason University, Manassas, Virginia, USA;School of Systems Biology, George Mason University, Manassas, Virginia, USA;National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, Virginia, USA;
关键词: Cranberry;    Proanthocyanidins;    Pseudomonas aeruginosa;    Biofilm;   
DOI  :  10.1186/1472-6882-14-499
 received in 2014-04-27, accepted in 2014-12-11,  发布年份 2014
来源: Springer
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【 摘 要 】

BackgroundBacteria within a biofilm are phenotypically more resistant to antibiotics, desiccation, and the host immune system, making it an important virulence factor for many microbes. Cranberry juice has long been used to prevent infections of the urinary tract, which are often related to biofilm formation. Recent studies have found that the A-type proanthocyanidins from cranberries have anti-biofilm properties against Escherichia coli.MethodsUsing crystal violet biofilm staining, resazurin metabolism assays, and confocal imaging, we examined the ability of A-type proanthocyanidins (PACs) to disrupt the biofilm formation of Pseudomonas aeruginosa. We used mass spectrometry to analyze the proteomic effects of PAC treatment. We also performed synergy assays and in vitro and in vivo infections to determine whether PACs, alone and in combination with gentamicin, could contribute to the killing of P. aeruginosa and the survival of cell lines and G. mellonella.ResultsCranberry PACs reduced P. aeruginosa swarming motility. Cranberry PACs significantly disrupted the biofilm formation of P. aeruginosa. Proteomics analysis revealed significantly different proteins expressed following PAC treatment. In addition, we found that PACs potentiated the antibiotic activity of gentamicin in an in vivo model of infection using G. mellonella.ConclusionsResults suggest that A-type proanthocyanidins may be a useful therapeutic against the biofilm-mediated infections caused by P. aeruginosa and should be further tested.

【 授权许可】

Unknown   
© Ulrey et al.; licensee BioMed Central. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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