BMC Complementary and Alternative Medicine | |
Cranberry proanthocyanidins have anti-biofilm properties against Pseudomonas aeruginosa | |
Monique L van Hoek3  Weidong Zhou1  Stephanie M Barksdale4  Robert K Ulrey2  | |
[1] Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, Virginia, USA;Department of Biology, George Mason University, Manassas, Virginia, USA;National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, Virginia, USA;School of Systems Biology, George Mason University, Manassas, Virginia, USA | |
关键词: Biofilm; Pseudomonas aeruginosa; Proanthocyanidins; Cranberry; | |
Others : 1084682 DOI : 10.1186/1472-6882-14-499 |
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received in 2014-04-27, accepted in 2014-12-11, 发布年份 2014 | |
【 摘 要 】
Background
Bacteria within a biofilm are phenotypically more resistant to antibiotics, desiccation, and the host immune system, making it an important virulence factor for many microbes. Cranberry juice has long been used to prevent infections of the urinary tract, which are often related to biofilm formation. Recent studies have found that the A-type proanthocyanidins from cranberries have anti-biofilm properties against Escherichia coli.
Methods
Using crystal violet biofilm staining, resazurin metabolism assays, and confocal imaging, we examined the ability of A-type proanthocyanidins (PACs) to disrupt the biofilm formation of Pseudomonas aeruginosa. We used mass spectrometry to analyze the proteomic effects of PAC treatment. We also performed synergy assays and in vitro and in vivo infections to determine whether PACs, alone and in combination with gentamicin, could contribute to the killing of P. aeruginosa and the survival of cell lines and G. mellonella.
Results
Cranberry PACs reduced P. aeruginosa swarming motility. Cranberry PACs significantly disrupted the biofilm formation of P. aeruginosa. Proteomics analysis revealed significantly different proteins expressed following PAC treatment. In addition, we found that PACs potentiated the antibiotic activity of gentamicin in an in vivo model of infection using G. mellonella.
Conclusions
Results suggest that A-type proanthocyanidins may be a useful therapeutic against the biofilm-mediated infections caused by P. aeruginosa and should be further tested.
【 授权许可】
2014 Ulrey et al.; licensee BioMed Central.
【 预 览 】
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