Comparative study of the antioxidant and reactive oxygen species scavenging properties in the extracts of the fruits of Terminalia chebula, Terminalia belerica and Emblica officinalis

BMC Complementary and Alternative Medicine

Research Article

Bibhabasu Hazra¹  Nripendranath Mandal¹  Rhitajit Sarkar¹  Santanu Biswas¹ 

[1] Division of Molecular Medicine, Bose Institute, P-1/12 CIT Scheme VIIM, 700054, Kolkata, India;

关键词: Curcumin;    Quercetin;    Scavenge Activity;    Gallic Acid;    Flavonoid Content;   

DOI  :  10.1186/1472-6882-10-20

 received in 2009-12-31, accepted in 2010-05-13,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundCellular damage caused by reactive oxygen species (ROS) has been implicated in several diseases, and hence natural antioxidants have significant importance in human health. The present study was carried out to evaluate the in vitro antioxidant and reactive oxygen species scavenging activities of Terminalia chebula, Terminalia belerica and Emblica officinalis fruit extracts.MethodsThe 70% methanol extracts were studied for in vitro total antioxidant activity along with phenolic and flavonoid contents and reducing power. Scavenging ability of the extracts for radicals like DPPH, hydroxyl, superoxide, nitric oxide, hydrogen peroxide, peroxynitrite, singlet oxygen, hypochlorous acid were also performed to determine the potential of the extracts.ResultsThe ability of the extracts of the fruits in exhibiting their antioxative properties follow the order T. chebula > E. officinalis > T. belerica. The same order is followed in their flavonoid content, whereas in case of phenolic content it becomes E. officinalis > T. belerica > T. chebula. In the studies of free radicals' scavenging, where the activities of the plant extracts were inversely proportional to their IC50 values, T. chebula and E. officinalis were found to be taking leading role with the orders of T. chebula > E. officinalis > T. belerica for superoxide and nitric oxide, and E. officinalis > T. belerica > T. chebula for DPPH and peroxynitrite radicals. Miscellaneous results were observed in the scavenging of other radicals by the plant extracts, viz., T. chebula > T. belerica > E. officinalis for hydroxyl, T. belerica > T. chebula > E. officinalis for singlet oxygen and T. belerica > E. officinalis > T. chebula for hypochlorous acid. In a whole, the studied fruit extracts showed quite good efficacy in their antioxidant and radical scavenging abilities, compared to the standards.ConclusionsThe evidences as can be concluded from the study of the 70% methanol extract of the fruits of Terminalia chebula, Terminalia belerica and Emblica officinalis, imposes the fact that they might be useful as potent sources of natural antioxidant.

【 授权许可】

Unknown   
© Hazra et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.