Bidirectional promoters in seed development and related hormone/stress responses

BMC Plant Biology

Research Article

Kate Lee¹  Pascale Rossignol²  Rachel Hemsley³  Thaleia Papageorgiou⁴  Sinéad Drea⁴  Sofia Kourmpetli⁴ 

[1] Bioinformatics and Biostatistics Analysis Support Hub (BBASH), College of Medicine, Biological Sciences and Psychology, University of Leicester, Leicester, UK;Current address Department of Molecular, Cellular and Developmental Biology, Yale University, 06520, New Haven, CT, USA;Current address UCL Business PLC, The Network Building, 97 Tottenham Court Road, W1T 4TP, London, UK;Department of Biology, University of Leicester, University Road, LE1 7RH, Leicester, UK;

关键词: Arabidopsis thaliana;    Bidirectional promoter;    G-box;    ABRE;    Abscisic acid;    Seed development;    Desiccation;    Stress response;   

DOI  :  10.1186/1471-2229-13-187

 received in 2013-07-16, accepted in 2013-11-15,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundBidirectional promoters are common in genomes but under-studied experimentally, particularly in plants. We describe a targeted identification and selection of a subset of putative bidirectional promoters to identify genes involved in seed development and to investigate possible coordinated responses of gene pairs to conditions important in seed maturation such as desiccation and ABA-regulation.ResultsWe combined a search for 100–600 bp intergenic regions in the Arabidopsis genome with a cis-element based selection for those containing multiple copies of the G-box motif, CACGTG. One of the putative bidirectional promoters identified also contained a CE3 coupling element 5 bp downstream of one G-box and is identical to that characterized previously in the HVA1 promoter of barley. CE3 elements are significantly under-represented and under-studied in Arabidopsis. We further characterized the pair of genes associated with this promoter and uncovered roles for two small, previously uncharacterized, plant-specific proteins in Arabidopsis seed development and stress responses.ConclusionsUsing bioinformatics we identified putative bidirectional promoters involved in seed development and analysed expression patterns for a pair of plant-specific genes in various tissues and in response to hormones/stress. We also present preliminary functional analysis of these genes that is suggestive of roles in seed development.

【 授权许可】

Unknown   
© Kourmpetli et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.