Structure and differential response to abscisic acid of two promoters for the cytosolic copper/zinc‐ superoxide dismutase genes, SodCcl and SodCc2, in rice protoplasts

FEBS Letters

Tanaka, Kunisuke¹  Sakamoto, Atsushi¹  Kaminaka, Hironori¹  Sumi, Kazuhiko¹  Okumura, Takekazu¹ 

[1] Department of Biochemistry, College of Agriculture, Kyoto Prefectural University, Shimogamo, Kyoto 606, Japan

关键词: Abscisic acid;    Differential gene regulation;    Inducible promoter;    Superoxide dismutase;    Cellular redox;    Rice;    ABA;    abscisic acid;    ABRE;    abscisic acid-responsive element;    -SOD;    copper/zinc-superoxide dismutase;    GA3;    gibberellin A3;    GUS;    β-glucuronidase;    SOD;    superoxide dismutase;    Sod;    superoxide dismutase gene;    SodCc2;    cytosolic copper/zinc-superoxide dismutase gene;   

DOI  :  10.1016/0014-5793(94)01396-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We determined the 5′-flanking sequences of two nuclear genes (SodCcl and SodCcl) encoding cytosolic copper/zinc-superoxide dismutase in rice (Oryza sativa L.). Utilizing transient β-glucuronidase (GUS) reporter assays, functional promoter—GUS analysis was performed in rice protoplasts exposed to the phytohormone abscisic acid (ABA) or the antioxidant sulfhydryl reagent, dithiothreitol (DTT). Transcriptional activities from both SodCc—GUS fusions were stimulated by DTT, which induces the promoter activity of the tobacco SodCc gene [Proc. Natl. Acad. Sci. USA 90 (1993) 3108–3112]. ABA had no effect on SodCcl—GUS expression but specifically induced the gene expression of the SodCcl—GUS fusion. The simultaneous application of ABA and gibberellin A3, however, abolished the enhancing effect of ABA. These results indicated that two rice SodCc promoters differentially respond to externally supplied ABA and that one of the regulatory factors for plant SodCc expression is ABA in addition to cellular redox-modulating antioxidants.

【 授权许可】

Unknown