Overexpression of members of the microRNA-183 family is a risk factor for lung cancer: A case control study

BMC Cancer

Research Article

Yong Kui Zhang¹  DongDong Chen²  XiaoGuang Liu²  JianYing He²  Wangyu Zhu²  YanYan Hunag² 

[1] Department of Cardio-Thoracic Surgery, Zhoushan Hospital of Zhejiang Province, 316004, 316004, Zhoushan, Zhejiang, China;Joint Laboratory of Immunogenomics, Zhoushan Hospital-BIG/CAS, 316004, Zhoushan, Zhejiang Province, China;

关键词: miRNA;    diagnosis;    prognosis;    sera;    RT-quantitative PCR;    human lung cancer;   

DOI  :  10.1186/1471-2407-11-393

 received in 2011-06-17, accepted in 2011-09-15,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundLung cancer is the leading cause of cancer-related deaths worldwide. Early detection is considered critical for lung cancer treatment. MicroRNAs (miRNAs) have shown promise as diagnostic and prognostic indicators. This study was to identify specific miRNAs with diagnostic and prognostic value for patients with lung cancer, and to explore the correlation between expression profiles of miRNAs and patient survival.MethodsGene expression of members of the miR-183 family (miR-96, miR-182, and miR-183) were examined in 70 paired samples from lung cancer patients (primary cancer and non-cancerous tissues and sera), as well as 44 serum samples from normal volunteers and lung cancer cell lines by quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR). The correlation between the expression of miRNAs in tissues, sera, and patient overall survival were also examined by log-rank and Cox regression analysis.ResultsExpression levels of members of the miR-183 family in lung cancer tumor and sera were higher than that of their normal counterparts. The miR-96 expression in tumors was positively associated with its expression in sera. Log-rank and Cox regression analyses demonstrated that high expression of tumor and serum miRNAs of the miR-183 family were associated with overall poor survival in patients with lung cancer.ConclusionsOur results suggest that the expressions of miR-96, miR-182, and miR-183 in tumor and sera may be considered potential novel biomarkers for the diagnosis and prognosis of lung cancer.

【 授权许可】

Unknown   
© Zhu et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.