Comparative transcriptional profiling of orange fruit in response to the biocontrol yeast Kloeckera apiculata and its active compounds

BMC Genomics

Research Article

Chao-an Long¹  Kai Chen¹  Guofeng Li²  Pu Liu³  Xiaoping Yang⁴ 

[1] Key Laboratory of Horticultural Plant Biology of the Ministry of Education, National Centre of Citrus Breeding, Huazhong Agricultural University, 430070, Wuhan, P. R. China;Key Laboratory of Pomology, Anhui Agricultural University, 230036, Hefei, P. R. China;Key Laboratory of Pomology, Anhui Agricultural University, 230036, Hefei, P. R. China;Key Laboratory of Horticultural Plant Biology of the Ministry of Education, National Centre of Citrus Breeding, Huazhong Agricultural University, 430070, Wuhan, P. R. China;Research Institute of Fruit and Tea, Hubei Academy of Agricultural Science, 430064, Wuhan, P. R. China;

关键词: Biological control;    Kloeckera apiculata;    Plant defence;    Postharvest;    Citrus;   

DOI  :  10.1186/s12864-015-2333-3

 received in 2015-01-31, accepted in 2015-12-18,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe yeast Kloeckera apiculata strain 34–9 is an antagonist that shows biological control activity against the postharvest fungal pathogens of citrus. An antifungal compound, 2-phenylethanol (PEA), has been identified from the extract of K. apiculata. To better understand the molecular processes underlying the response of citrus fruit tissue to K. apiculata, the extract and PEA, microarray analyses were performed on navel oranges using an Affymetrix Citrus GeneChip.ResultsAs many as 801, 339 and 608 differentially expressed genes (DEGs) were identified after the application of K. apiculata, the extract and PEA, respectively. In general, K. apiculata induced the expression of defence-related genes. In addition to chitinase and β-1,3-glucanase, genes involved in ethylene (ET), jasmonic acid (JA), calcium signalling, MAPK signalling and phenylalanine metabolism were induced. In contrast, monodehydroascorbate reductase, superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and carotenoid biosynthesis genes were down-regulated. The expression profiles for the extract- and PEA-treated samples were similar to that found for yeast (sharing 57.4 % DEGs), with a significant increase in the transcript levels of defence-related genes.ConclusionThis study provides a global picture of the gene expression changes in navel oranges after the application of the antagonist yeast K. apiculata, its extract and PEA. The interpretation of the DEGs revealed new insight into the molecular processes that regulate the defence responses in orange tissue.

【 授权许可】

CC BY   
© Liu et al. 2016