Mechanisms of action for 2-phenylethanol isolated from Kloeckera apiculata in control of Penicillium molds of citrus fruits

BMC Microbiology

Xiuxin Deng³  Chao-an Long³  Kai Chen³  Yujia Liu³  Meng Yang¹  Yunjiang Cheng³  Pu Liu² 

[1] Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Chaoyang District, Beijing 100029, P. R. China;Key Laboratory of Pomology, Anhui Agricultural University, Hefei 230036, P. R. China;Key Laboratory of Horticultural Plant Biology of the Ministry of Education, National Centre of Citrus Breeding, Huazhong Agricultural University, Wuhan 430070, P. R. China

关键词: Postharvest;    2-phenylethanol (PEA);    Antifungal compound;    Kloeckera apiculata;    Penicillium;    Biological control;   

Others  :  1170532
DOI  :  10.1186/s12866-014-0242-2

 received in 2013-10-27, accepted in 2014-09-08,  发布年份 2014

【 摘 要 】

Background

Green and blue mold decay, caused by Penicillium digitatum and P. italicum, respectively, are important postharvest diseases of citrus. Biocontrol by microbes is an alternative to synthetic fungicide application. In this study, the antagonistic yeast strain Kloeckera apiculata 34¿9 was used to investigate the action mechanisms involved in the biocontrol of postharvest diseases.

Results

An antifungal substance, 2-phenylethanol (PEA), was isolated from K. apiculata and demonstrated to have antimicrobial activity against selected phytopathogenic fungi. Experiments on P. italicum cells identified the mitochondria and the nucleus as particularly sensitive to inhibition. Regulation of P. italicum gene expression was investigated using RNA-Seq. PEA up-regulated genes involved with the peroxisome, regulation of autophagy, phosphatidylinositol signaling system, protein processing in endoplasmic reticulum, fatty acid metabolism, and inhibited ribosome, RNA polymerase, DNA replication, amino acid biosynthesis, aminoacyl-tRNA biosynthesis and cell cycle. Inhibitory responses revealed by RNA-Seq suggest that PEA might compete for attachment on the active site of phenylalanyl-tRNA synthetase (PheRS).

Conclusion

This study provided new insight on the mode of action of biocontrol yeast agents in controlling postharvest pathogenic fungi.

【 授权许可】

   
2014 Liu et al.; licensee BioMed Central Ltd.

【 图 表 】

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