Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway

BMC Complementary and Alternative Medicine

Research Article

Cheng-Chen Hsu¹  Mei-Chich Hsu²  Tzu-Shao Yeh³  Suh-Ching Yang³  Jen-Fang Liu⁴ 

[1] Department of Anatomy, School of Medicine, Taipei Medical University, 11031, Taipei, Taiwan;Department of Sports Medicine, Kaohsiung Medical University, 80708, Kaohsiung, Taiwan;Graduate Institute of Sports Science, National Taiwan Sport University, 33301, Taoyuan, Taiwan;School of Nutrition and Health Sciences, Taipei Medical University, 11031, Taipei, Taiwan;School of Nutrition and Health Sciences, Taipei Medical University, 11031, Taipei, Taiwan;Department of Nutrition and Health Sciences, Chang Gung University of Science and Technology, 33303, Taoyuan, Taiwan;Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, 33303, Taoyuan, Taiwan;

关键词: C2C12;    Dong Quai;    Muscle;    IGF-1;   

DOI  :  10.1186/1472-6882-14-144

 received in 2013-09-29, accepted in 2014-03-25,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundAngelica Sinensis (AS), a folk medicine, has long been used in ergogenic aids for athletes, but there is little scientific evidence supporting its effects. We investigated whether AS induces hypertrophy in myotubes through the phosphatidylinositol 3-kinase (PI3K)/Akt (also termed PKB)/mammalian target of the rapamycin (mTOR) pathway.MethodsAn in vitro experiment investigating the induction of hypertrophy in myotubes was conducted. To investigate whether AS promoted the hypertrophy of myotubes, an established in vitro model of myotube hypertrophy with and without AS was used and examined using microscopic images. The role of the PI3K/Akt/mTOR signaling pathway in AS-induced myotube hypertrophy was evaluated. Two inhibitors, wortmannin (an inhibitor of PI3K) and rapamycin (an inhibitor of mTOR), were used.ResultThe results revealed that the myotube diameters in the AS-treated group were significantly larger than those in the untreated control group (P < 0.05). Wortmannin and rapamycin inhibited AS-induced hypertrophy. Furthermore, AS increased Akt and mTOR phosphorylation through the PI3K pathway and induced myotube hypertrophy.ConclusionThe results confirmed that AS induces hypertrophy in myotubes through the PI3K/Akt/mTOR pathway.

【 授权许可】

Unknown   
© Yeh et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.