Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway

BMC Complementary and Alternative Medicine

Jen-Fang Liu²  Mei-Chich Hsu³  Suh-Ching Yang⁴  Cheng-Chen Hsu¹  Tzu-Shao Yeh⁴ 

[1] Department of Anatomy, School of Medicine, Taipei Medical University, Taipei 11031, Taiwan;Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 33303, Taiwan;Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan;School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, Taiwan

关键词: IGF-1;    Muscle;    Dong Quai;    C2C12;   

Others  :  1220176
DOI  :  10.1186/1472-6882-14-144

 received in 2013-09-29, accepted in 2014-03-25,  发布年份 2014

【 摘 要 】

Background

Angelica Sinensis (AS), a folk medicine, has long been used in ergogenic aids for athletes, but there is little scientific evidence supporting its effects. We investigated whether AS induces hypertrophy in myotubes through the phosphatidylinositol 3-kinase (PI3K)/Akt (also termed PKB)/mammalian target of the rapamycin (mTOR) pathway.

Methods

An in vitro experiment investigating the induction of hypertrophy in myotubes was conducted. To investigate whether AS promoted the hypertrophy of myotubes, an established in vitro model of myotube hypertrophy with and without AS was used and examined using microscopic images. The role of the PI3K/Akt/mTOR signaling pathway in AS-induced myotube hypertrophy was evaluated. Two inhibitors, wortmannin (an inhibitor of PI3K) and rapamycin (an inhibitor of mTOR), were used.

Result

The results revealed that the myotube diameters in the AS-treated group were significantly larger than those in the untreated control group (P < 0.05). Wortmannin and rapamycin inhibited AS-induced hypertrophy. Furthermore, AS increased Akt and mTOR phosphorylation through the PI3K pathway and induced myotube hypertrophy.

Conclusion

The results confirmed that AS induces hypertrophy in myotubes through the PI3K/Akt/mTOR pathway.

【 授权许可】

   
2014 Yeh et al.; licensee BioMed Central Ltd.

【 图 表 】

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