A putative Chondroprotective role for IL-1β and MPO in herbal treatment of experimental osteoarthritis

BMC Complementary and Alternative Medicine

Research Article

Nora M. Aborehab¹  Abeer Refaiy²  Mahitab H. El Bishbishy³  Nermien E. Waly⁴ 

[1] Department of Biochemistry, Faculty of Pharmacy, MSA University, 11787, Giza, Egypt;Department of Pathology Faculty of Medicine, Assiut University, 71515, Assiut, Egypt;Department of Pharmacognosy, Faculty of Pharmacy, MSA University, 11787, Giza, Egypt;Department of Physiology, Faculty of Medicine, Helwan University, Cairo, 11795, Egypt;

关键词: Ginger;    Curcumin;    Osteoarthritis;    IL-1β;    COMP;    HA;    MPO;    MDA;   

DOI  :  10.1186/s12906-017-2002-y

 received in 2017-05-29, accepted in 2017-11-14,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundHerbal treatment may have a chondroprotective and therapeutic effect on Osteoarthritis (OA). We investigated the mechanism of action of ginger and curcumin rhizomes cultivated in Egypt in treatment of OA in rat model.MethodsThirty-five albino rats were intra-articularly injected with Monosodium Iodoacetate in the knee joint. Ginger and curcumin was orally administered at doses of 200 and 400 mg/kg (F200 and F400). Serum levels of cartilage oligomeric matrix protein (COMP), hyaluronic acid (HA), malondialdehyde (MDA), myeloperoxidase (MPO), Interleukin-1 beta (IL-1β) and superoxide dismutase activity (SOD) were measured using ELISA. The composition of the herbal formula hydro-ethanolic extract was characterized using UPLC-ESI-MS. Histopathological changes in injected joints was examined using routine histopathology. Statistical analysis was performed using one-way ANOVA.ResultsSerum levels of COMP, HA, MPO, MDA, and IL-1β were significantly decreased in F 200, F 400 and V groups when compared to OA group (P value <0.0001). On the other hand SOD levels were significantly elevated in treated groups compared to OA groups (P value <0.0001).ConclusionsThe ginger/curcumin at 1:1 had chondroprotective effect via anti-inflammatory and antioxidant effect in rat OA model. Further pharmacological and clinical studies are needed to evaluate this effect.

【 授权许可】

CC BY   
© The Author(s). 2017