Castanea sativa Mill. bark extract exhibits chemopreventive properties triggering extrinsic apoptotic pathway in Jurkat cells

BMC Complementary and Alternative Medicine

Research Article

Silvana Hrelia¹  Marco Malaguti¹  Veronica Cocchi²  Patrizia Hrelia²  Monia Lenzi² 

[1] Department for Life Quality Studies, University of Bologna, Corso d’Augusto 237, 47921, Rimini, Italy;Department of Pharmacy and Biotechnology, University of Bologna, Via San Donato 15, 40127, Bologna, Italy;

关键词: Castanea sativa;    Sweet chestnut;    Cytotoxicity;    Apoptosis;    Chemoprevention;    Flow cytometry;    Jurkat cells;   

DOI  :  10.1186/s12906-017-1756-6

 received in 2016-11-10, accepted in 2017-04-26,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundChemoprevention represents the possibility to prevent, stop or reverse the cancerogenetic process. In this context the interest towards natural extracts and botanical drugs has constantly grown due to their phytochemical content. Castanea sativa Mill. (CSM) extracts showed to exert positive effect in the prevention/counteraction of chronic/degenerative diseases, therefore, we evaluated the potential chemopreventive effect of CSM bark extract.MethodsFlow cytometry (FCM) analyses of Jurkat cells treated with CSM bark extract (0–500 μg·mL−1) for 24–72 h allowed evaluating its cytotoxicity and ability to induce apoptosis through the intrinsic or extrinsic pathways. Moreover, to evaluate CSM bark extract selectivity towards cancer cells, its cytotoxic and pro-apoptotic effect was also evaluated in human peripheral blood lymphocytes (PBL).ResultsCSM bark extract induced apoptosis in Jurkat cells in a dose- and time- dependent manner activating the extrinsic pathways as evidenced by the increase of activated caspase-8 positive cells. Moreover, IC50 calculated after 24 h treatment resulted 304 and 128 μg·mL−1 in PBL and Jurkat cells respectively.ConclusionsOur data suggest that CSM bark extract might be considered an interesting potential anti-cancer agent, since it induces apoptosis in cancer cells without appreciable cytotoxic effects on non-transformed cells.

【 授权许可】

CC BY   
© The Author(s). 2017