Is cytotoxicity a determinant of the different in vitro and in vivo effects of bioactives?

BMC Complementary and Alternative Medicine

Research Article

Veronica Valli¹  Francesca Danesi¹  Lucía Murgui-Bosch²  Teresa Tomás-Chisbert²  Lidia Tomás-Cobos²  Mattia Di Nunzio³  Alessandra Bordoni⁴ 

[1] Department of Agri-Food Sciences and Technologies, University of Bologna, Piazza Goidanich 60, 47521, Cesena, Italy;Department of Bioassays, Ainia Centro Tecnológico, Parque Tecnológico de Valencia, c. Benjamin Franklin 5-11, E46980, Paterna, Valencia, Spain;Interdepartmental Centre for Industrial Agri-Food Research, University of Bologna, Piazza Goidanich 60, 47521, Cesena, Italy;Interdepartmental Centre for Industrial Agri-Food Research, University of Bologna, Piazza Goidanich 60, 47521, Cesena, Italy;Department of Agri-Food Sciences and Technologies, University of Bologna, Piazza Goidanich 60, 47521, Cesena, Italy;

关键词: Cytotoxicity;    Docosahexaenoic acid;    O;    Protocatechuic acid;    Propionate;    HepG2 cells;   

DOI  :  10.1186/s12906-017-1962-2

 received in 2017-06-01, accepted in 2017-09-01,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundFoodstuffs of both plant and animal origin contain a wide range of bioactive compounds. Although human intervention studies are mandatory to assess the health effects of bioactives, the in vitro approach is often used to select the most promising molecules to be studied in vivo. To avoid misleading results, concentration and chemical form, exposure time, and potential cytotoxicity of the tested bioactives should be carefully set prior to any other experiments.MethodsIn this study the possible cytotoxicity of different bioactives (docosahexaenoic acid, propionate, cyanidin-3-O-glucoside, protocatechuic acid), was investigated in HepG2 cells using different methods. Bioactives were supplemented to cells at different concentrations within the physiological range in human blood, alone or in combination, considering two different exposure times.ResultsReported data clearly evidence that in vitro cytotoxicity is tightly related to the exposure time, and it varies among bioactives, which could exert a cytotoxic effect even at a concentration within the in vivo physiological blood concentration range. Furthermore, co-supplementation of different bioactives can increase the cytotoxic effect.ConclusionsOur results underline the importance of in vitro cytotoxicity screening that should be considered mandatory before performing studies aimed to evaluate the effect of bioactives on other cellular parameters. Although this study is far from the demonstration of a toxic effect of the tested bioactives when administered to humans, it represents a starting point for future research aimed at verifying the existence of a potential hazard due to the wide use of high doses of multiple bioactives.

【 授权许可】

CC BY   
© The Author(s). 2017