Trichoderma viride cellulase induces resistance to the antibiotic pore-forming peptide alamethicin associated with changes in the plasma membrane lipid composition of tobacco BY-2 cells

BMC Plant Biology

Research Article

Henrik Stålbrand¹  Mari Aidemark²  Allan G Rasmusson²  Susanne Widell²  Erik Andreasson³  Anna Stina Sandelius⁴  Henrik Tjellström⁵ 

[1] Department of Biochemistry, P.O. Box 124, SE-221 00, Lund, Sweden;Department of Biology, Lund University, Sölvegatan 35, SE-223 62, LUND, Sweden;Department of Plant Protection Biology, Swedish Agricultural University, P.O. Box 102, SE-230 53, Alnarp, Sweden;Department of Plant and Environmental Sciences, Göteborg University, P.O. Box 461, SE-405 30, Göteborg, Sweden;Plant Biology Department, Michigan State University, 48824, East Lansing, MI, USA;Department of Plant and Environmental Sciences, Göteborg University, P.O. Box 461, SE-405 30, Göteborg, Sweden;

关键词: Cellulase;    Cellobiose;    Phosphatidic Acid;    Microsomal Fraction;    Tobacco Cell;   

DOI  :  10.1186/1471-2229-10-274

 received in 2010-09-09, accepted in 2010-12-14,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundAlamethicin is a membrane-active peptide isolated from the beneficial root-colonising fungus Trichoderma viride. This peptide can insert into membranes to form voltage-dependent pores. We have previously shown that alamethicin efficiently permeabilises the plasma membrane, mitochondria and plastids of cultured plant cells. In the present investigation, tobacco cells (Nicotiana tabacum L. cv Bright Yellow-2) were pre-treated with elicitors of defence responses to study whether this would affect permeabilisation.ResultsOxygen consumption experiments showed that added cellulase, already upon a limited cell wall digestion, induced a cellular resistance to alamethicin permeabilisation. This effect could not be elicited by xylanase or bacterial elicitors such as flg22 or elf18. The induction of alamethicin resistance was independent of novel protein synthesis. Also, the permeabilisation was unaffected by the membrane-depolarising agent FCCP. As judged by lipid analyses, isolated plasma membranes from cellulase-pretreated tobacco cells contained less negatively charged phospholipids (PS and PI), yet higher ratios of membrane lipid fatty acid to sterol and to protein, as compared to control membranes.ConclusionWe suggest that altered membrane lipid composition as induced by cellulase activity may render the cells resistant to alamethicin. This induced resistance could reflect a natural process where the plant cells alter their sensitivity to membrane pore-forming agents secreted by Trichoderma spp. to attack other microorganisms, and thus adding to the beneficial effect that Trichoderma has for plant root growth. Furthermore, our data extends previous reports on artificial membranes on the importance of lipid packing and charge for alamethicin permeabilisation to in vivo conditions.

【 授权许可】

Unknown   
© Aidemark et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.