Selecting β-glucosidases to support cellulases in cellulose saccharification

Biotechnology for Biofuels

Hele Teugjas¹  Priit Väljamäe¹ 

[1] Institute of Molecular and Cell Biology, University of Tartu, Riia 23b – 202, 51010, Tartu, Estonia

关键词: Thermoascus aurantiacus;    Acremonium thermophilum;    Inhibition;    Glucose;    Cellobiose;    β-glucosidase;    Cellulose;    Cellulase;   

Others  :  797969
DOI  :  10.1186/1754-6834-6-105

 received in 2013-05-20, accepted in 2013-07-11,  发布年份 2013

【 摘 要 】

Background

Enzyme end-product inhibition is a major challenge in the hydrolysis of lignocellulose at a high dry matter consistency. β-glucosidases (BGs) hydrolyze cellobiose into two molecules of glucose, thereby relieving the product inhibition of cellobiohydrolases (CBHs). However, BG inhibition by glucose will eventually lead to the accumulation of cellobiose and the inhibition of CBHs. Therefore, the kinetic properties of candidate BGs must meet the requirements determined by both the kinetic properties of CBHs and the set-up of the hydrolysis process.

Results

The kinetics of cellobiose hydrolysis and glucose inhibition of thermostable BGs from Acremonium thermophilum (AtBG3) and Thermoascus aurantiacus (TaBG3) was studied and compared to Aspergillus sp. BG purified from Novozyme®188 (N188BG). The most efficient cellobiose hydrolysis was achieved with TaBG3, followed by AtBG3 and N188BG, whereas the enzyme most sensitive to glucose inhibition was AtBG3, followed by TaBG3 and N188BG. The use of higher temperatures had an advantage in both increasing the catalytic efficiency and relieving the product inhibition of the enzymes. Our data, together with data from a literature survey, revealed a trade-off between the strength of glucose inhibition and the affinity for cellobiose; therefore, glucose-tolerant BGs tend to have low specificity constants for cellobiose hydrolysis. However, although a high specificity constant is always an advantage, in separate hydrolysis and fermentation, the priority may be given to a higher tolerance to glucose inhibition.

Conclusions

The specificity constant for cellobiose hydrolysis and the inhibition constant for glucose are the most important kinetic parameters in selecting BGs to support cellulases in cellulose hydrolysis.

【 授权许可】

   
2013 Teugjas and Väljamäe; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Scheme 1

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