Multilocus characterization and phylogenetic analysis of Leishmania siamensisisolated from autochthonous visceral leishmaniasis cases, southern Thailand

BMC Microbiology

Research Article

Hirotomo Kato¹  Peerapan Tan-ariya²  Atitaya Hitakarun²  Padet Siriyasatien³  Saovanee Leelayoova⁴  Mathirut Mungthin⁴  Suradej Siripattanapipong⁴  Seksit Osatakul⁵ 

[1] Department of Disease Control, Laboratory of Parasitology, Graduate School of Veterinary Medicine, Hokkaido University, 060-0818, Sapporo, Japan;Department of Microbiology, Faculty of Science, Mahidol University, 10400, Bangkok, Thailand;Department of Parasitology, Faculty of Medicine, Chulalongkorn University, 10330, Bangkok, Thailand;Department of Parasitology, Phramongkutklao College of Medicine, 10400, Bangkok, Thailand;Department of Pediatrics, Faculty of Medicine, Prince of Songkla University, 90110, Songkhla, Thailand;

关键词: Leishmania siamensis;    Phylogenetic analysis;    Multilocus characterization;    Thailand;    Lineage;   

DOI  :  10.1186/1471-2180-13-60

 received in 2012-09-06, accepted in 2013-03-13,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundVisceral leishmaniasis (VL) caused by Leishmania siamensis is an emerging disease continuously reported in six southern provinces of Thailand. To date, the phylogenetic relationships among Leishmania isolates from Thai patients and other Leishmania species are still unclear and the taxonomic diversity needs to be established. In this study, the phylogenetic inference trees were constructed based on four genetic loci (i.e., SSU-rRNA, ITS1, hsp70, and cyt b), using DNA sequences obtained from autochthonous VL patients from southern Thailand and reference sequences of reported Leishmania isolates from other studies deposited in GenBank.ResultsPhylogenetic analyses of hsp70 and cyt b loci supported a clade comprised of L. siamensis isolates, which is independent to the other members in the genus Leishmania. In combination with genetic distance analysis, sequence polymorphisms were observed among L. siamensis isolates and two different lineages could be differentiated, lineages PG and TR. Phylogenetic analysis of the cyt b gene further showed that L. siamensis lineage TR is closely related to L. enrietti, a parasite of guinea pigs.ConclusionThe finding of this study sheds further light on the relationships of L. siamensis, both in intra- and inter-species aspects. This information would be useful for further in-depth studies on the biological properties of this important parasite.

【 授权许可】

CC BY   
© Leelayoova et al.; licensee BioMed Central Ltd. 2013