Multilocus characterization and phylogenetic analysis of Leishmania siamensis isolated from autochthonous visceral leishmaniasis cases, southern Thailand

BMC Microbiology

Mathirut Mungthin²  Seksit Osatakul³  Padet Siriyasatien¹  Peerapan Tan-ariya⁴  Hirotomo Kato⁵  Atitaya Hitakarun⁴  Suradej Siripattanapipong²  Saovanee Leelayoova² 

[1] Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand;Department of Parasitology, Phramongkutklao College of Medicine, Bangkok 10400, Thailand;Department of Pediatrics, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, Thailand;Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand;Department of Disease Control, Laboratory of Parasitology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan

关键词: Lineage;    Thailand;    Multilocus characterization;    Phylogenetic analysis;    Leishmania siamensis;   

Others  :  1144206
DOI  :  10.1186/1471-2180-13-60

 received in 2012-09-06, accepted in 2013-03-13,  发布年份 2013

【 摘 要 】

Background

Visceral leishmaniasis (VL) caused by Leishmania siamensis is an emerging disease continuously reported in six southern provinces of Thailand. To date, the phylogenetic relationships among Leishmania isolates from Thai patients and other Leishmania species are still unclear and the taxonomic diversity needs to be established. In this study, the phylogenetic inference trees were constructed based on four genetic loci (i.e., SSU-rRNA, ITS1, hsp70, and cyt b), using DNA sequences obtained from autochthonous VL patients from southern Thailand and reference sequences of reported Leishmania isolates from other studies deposited in GenBank.

Results

Phylogenetic analyses of hsp70 and cyt b loci supported a clade comprised of L. siamensis isolates, which is independent to the other members in the genus Leishmania. In combination with genetic distance analysis, sequence polymorphisms were observed among L. siamensis isolates and two different lineages could be differentiated, lineages PG and TR. Phylogenetic analysis of the cyt b gene further showed that L. siamensis lineage TR is closely related to L. enrietti, a parasite of guinea pigs.

Conclusion

The finding of this study sheds further light on the relationships of L. siamensis, both in intra- and inter-species aspects. This information would be useful for further in-depth studies on the biological properties of this important parasite.

【 授权许可】

   
2013 Leelayoova et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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