A reference gene set for sex pheromone biosynthesis and degradation genes from the diamondback moth, Plutella xylostella, based on genome and transcriptome digital gene expression analyses

BMC Genomics

Research Article

Ling-Hua Zuo¹  Yun-Fei Zhang²  Xing-Liang Wang³  Xian-Fu Tang⁴  Ming He⁵  Peng He⁵  Jun Wang⁵  Wei-Ming Xu⁵  Duan-Yang Hong⁶ 

[1] Agriculture Economic and Rural Development, RENMIN University of China, 100872, Beijing, People’s Republic of China;Biogas Institute of Ministry of Agriculture, 610041, Chengdu, People’s Republic of China;College of Plant Protection, Nanjing Agricultural University, 210095, Nanjing, People’s Republic of China;Guizhou Grass Jelly Biotechnology Company Limited, 564700, Chishui, Zhunyi, People’s Republic of China;State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, 550025, Guiyang, People’s Republic of China;The High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Druggability, Guizhou Medical University, Huaxi university town, Guian new district, 550025, Guizhou, People’s Republic of China;

关键词: Pheromone-biosynthesis enzymes;    Pheromone-degrading enzymes;    Detoxification;    Carboxyl/Cholinesterase;    Aldehyde oxidase;    Fatty acyl reductase;    Desaturase;   

DOI  :  10.1186/s12864-017-3592-y

 received in 2016-09-13, accepted in 2017-02-14,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundFemale moths synthesize species-specific sex pheromone components and release them to attract male moths, which depend on precise sex pheromone chemosensory system to locate females. Two types of genes involved in the sex pheromone biosynthesis and degradation pathways play essential roles in this important moth behavior. To understand the function of genes in the sex pheromone pathway, this study investigated the genome-wide and digital gene expression of sex pheromone biosynthesis and degradation genes in various adult tissues in the diamondback moth (DBM), Plutella xylostella, which is a notorious vegetable pest worldwide.ResultsA massive transcriptome data (at least 39.04 Gb) was generated by sequencing 6 adult tissues including male antennae, female antennae, heads, legs, abdomen and female pheromone glands from DBM by using Illumina 4000 next-generation sequencing and mapping to a published DBM genome. Bioinformatics analysis yielded a total of 89,332 unigenes among which 87 transcripts were putatively related to seven gene families in the sex pheromone biosynthesis pathway. Among these, seven [two desaturases (DES), three fatty acyl-CoA reductases (FAR) one acetyltransferase (ACT) and one alcohol dehydrogenase (AD)] were mainly expressed in the pheromone glands with likely function in the three essential sex pheromone biosynthesis steps: desaturation, reduction, and esterification. We also identified 210 odorant-degradation related genes (including sex pheromone-degradation related genes) from seven major enzyme groups. Among these genes, 100 genes are new identified and two aldehyde oxidases (AOXs), one aldehyde dehydrogenase (ALDH), five carboxyl/cholinesterases (CCEs), five UDP-glycosyltransferases (UGTs), eight cytochrome P450 (CYP) and three glutathione S-transferases (GSTs) displayed more robust expression in the antennae, and thus are proposed to participate in the degradation of sex pheromone components and plant volatiles.ConclusionsTo date, this is the most comprehensive gene data set of sex pheromone biosynthesis and degradation enzyme related genes in DBM created by genome- and transcriptome-wide identification, characterization and expression profiling. Our findings provide a basis to better understand the function of genes with tissue enriched expression. The results also provide information on the genes involved in sex pheromone biosynthesis and degradation, and may be useful to identify potential gene targets for pest control strategies by disrupting the insect-insect communication using pheromone-based behavioral antagonists.

【 授权许可】

CC BY   
© The Author(s). 2017