Degradation of glutathione S‐conjugates by a carboxypeptidase in the plant vacuole

FEBS Letters

Schröder, P.¹  Dietz, K.-J.²  Wolf, Andreas E.¹ 

[1] Fraunhofer Institut für atmosphärische Umwelforschung, Kreuzeckbahnstr. 19, D-82453 Garmisch-Partenkirchen, Germany;Julius-von-Sachs-Institut für Biowissenschaften, Universität Würzburg, Mittlerer Dallenbergweg 64, D-97082 Würzburg, Germany

关键词: Detoxification;    Glutathione conjugate;    Xenobiotic;    Carboxypeptidase;    Vacuole;   

DOI  :  10.1016/0014-5793(96)00272-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

For plants, glutathione conjugation is a major pathway to detoxify organic xenobiotic. Glutathione S-conjugates (SG-conjugates) are formed in the cytosol, the in vitro transport over the tonoplast has been described and a final storage in the vacuole has been postulated. We show here that alachlor rapidly accumulates as GS-conjugates in the plant vacuole and that the first step of its degradation, the formation of the respective γ-glutamylcysteinyl-S-conjugate, is catalyzed by a vacuolar carboxypeptidase. These results suggest the glutathione conjugate as a transport form but not a storage form of xenobiotic molecules.

【 授权许可】

Unknown