Gastric cancer cell supernatant causes apoptosis and fibrosis in the peritoneal tissues and results in an environment favorable to peritoneal metastases, in vitro and in vivo

BMC Gastroenterology

Research Article

Feng Li¹  Fu-Nan Liu²  Cheng-Gang Jiang²  Zhe Sun²  Yan Xu²  Hui-Mian Xu²  Di Na²  Zhi-Dong Lv²  Zhi-Feng Miao² 

[1] Department of Cell Biology, China Medical University, 110001, Shenyang, Liaoning Province, China;Department of Oncology, The First Affiliated Hospital, China Medical University, 110001, Shenyang, Liaoning Province, China;

关键词: Peritoneal carcinomatosis;    Stomach neoplasms;    Mesothelial cell;    Apoptosis;    Fibrosis;   

DOI  :  10.1186/1471-230X-12-34

 received in 2011-07-04, accepted in 2012-04-20,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundIn this study, we examined effects of soluble factors released by gastric cancer cells on peritoneal mesothelial cells in vitro and in vivo.MethodsHMrSV5, a human peritoneal mesothelial cell line, was incubated with supernatants from gastric cancer cells. Morphological changes of HMrSV5 cells were observed. Apoptosis of HMrSV5 cells was observed under a transmission electron microscope and quantitatively determined by MTT assay and flow cytometry. Expressions of apoptosis-related proteins (caspase-3, caspase-8, Bax, bcl-2) were immunochemically evaluated.ResultsConspicuous morphological changes indicating apoptosis were observed in HMrSV5 cells 24 h after treatment with the supernatants of gastric cancer cells. In vivo, peritoneal tissues treated with gastric cancer cell supernatant were substantially thickened and contained extensive fibrosis.ConclusionsThese findings demonstrate that supernatants of gastric cancer cells can induce apoptosis and fibrosis in HMrSV5 human peritoneal mesothelial cells through supernatants in the early peritoneal metastasis, in a time-dependent manner, and indicate that soluble factors in the peritoneal cavity affect the morphology and function of mesothelial cells so that the resulting environment can become favorable to peritoneal metastases.

【 授权许可】

Unknown   
© Na et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.