期刊论文详细信息
BMC Gastroenterology
Gastric cancer cell supernatant causes apoptosis and fibrosis in the peritoneal tissues and results in an environment favorable to peritoneal metastases, in vitro and in vivo
Research Article
Feng Li1  Fu-Nan Liu2  Cheng-Gang Jiang2  Zhe Sun2  Yan Xu2  Hui-Mian Xu2  Di Na2  Zhi-Dong Lv2  Zhi-Feng Miao2 
[1] Department of Cell Biology, China Medical University, 110001, Shenyang, Liaoning Province, China;Department of Oncology, The First Affiliated Hospital, China Medical University, 110001, Shenyang, Liaoning Province, China;
关键词: Peritoneal carcinomatosis;    Stomach neoplasms;    Mesothelial cell;    Apoptosis;    Fibrosis;   
DOI  :  10.1186/1471-230X-12-34
 received in 2011-07-04, accepted in 2012-04-20,  发布年份 2012
来源: Springer
PDF
【 摘 要 】

BackgroundIn this study, we examined effects of soluble factors released by gastric cancer cells on peritoneal mesothelial cells in vitro and in vivo.MethodsHMrSV5, a human peritoneal mesothelial cell line, was incubated with supernatants from gastric cancer cells. Morphological changes of HMrSV5 cells were observed. Apoptosis of HMrSV5 cells was observed under a transmission electron microscope and quantitatively determined by MTT assay and flow cytometry. Expressions of apoptosis-related proteins (caspase-3, caspase-8, Bax, bcl-2) were immunochemically evaluated.ResultsConspicuous morphological changes indicating apoptosis were observed in HMrSV5 cells 24 h after treatment with the supernatants of gastric cancer cells. In vivo, peritoneal tissues treated with gastric cancer cell supernatant were substantially thickened and contained extensive fibrosis.ConclusionsThese findings demonstrate that supernatants of gastric cancer cells can induce apoptosis and fibrosis in HMrSV5 human peritoneal mesothelial cells through supernatants in the early peritoneal metastasis, in a time-dependent manner, and indicate that soluble factors in the peritoneal cavity affect the morphology and function of mesothelial cells so that the resulting environment can become favorable to peritoneal metastases.

【 授权许可】

Unknown   
© Na et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

【 预 览 】
附件列表
Files Size Format View
RO202311096507612ZK.pdf 1127KB PDF download
【 参考文献 】
  • [1]
  • [2]
  • [3]
  • [4]
  • [5]
  • [6]
  • [7]
  • [8]
  • [9]
  • [10]
  • [11]
  • [12]
  • [13]
  • [14]
  • [15]
  • [16]
  • [17]
  • [18]
  • [19]
  • [20]
  • [21]
  • [22]
  • [23]
  • [24]
  • [25]
  • [26]
  • [27]
  • [28]
  • [29]
  文献评价指标  
  下载次数:4次 浏览次数:1次