Regulation of podocalyxin expression in the kidney of streptozotocin-induced diabetic rats with Chinese herbs (Yishen capsule)

BMC Complementary and Alternative Medicine

Research Article

Wenyuan Liu¹  Ruihua Wang¹  Yanyan Sun¹  Xiaodong Zhang¹  Jingai Fang¹  Qintao Chang¹  Hongkun Wei¹  Yuewen Gong² 

[1] Department of Nephrology, The First Affiliated Hospital to the Shanxi Medical University, 56 Xinjian South Road, 030001, Taiyuan, Shanxi Province, People’s Republic of China;Faculty of Pharmacy, University of Manitoba, R3E 0 T5, Winnipeg, Canada;

关键词: Diabetic Nephropathy;    Glomerular Basement Membrane;    Benazepril;    Slit Diaphragm;    Idiopathic Membranous Nephropathy;   

DOI  :  10.1186/1472-6882-13-76

 received in 2012-10-11, accepted in 2013-03-27,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundDiabetic nephropathy is an emergent issue in China with increase in patients with type II diabetes. There are several successful Chinese herbal products for the treatment of patients with diabetic nephropathy in China. However, the mechanisms mediating the biological activity of these products are still unclear. Podocalyxin is a sialoprotein critical to maintaining integrity of filtration function of glomerulus.MethodsBy employing streptozotocin-induced diabetic rats and a Chinese herb formulation (Yishen capsule), we examined the regulation of podocalyxin expression in the kidney by Yishen capsule through immunofluorescent staining and reverse transcriptase polymerase chain reaction.ResultsAfter injection of STZ, there were significant increase in both blood glucose and urinary protein. Serum creatinine and BUN were also increased in rats with injection of STZ. Moreover, expression of podocalyxin in the glomerulus was gradually reduced after injection of STZ. There was also a loss of podocyte foot processes in the glomerular basement membrane. However, Yishen capsule or benazepril was able to restore the expression of podocalyxin and podocyte foot processes in the kidney. Although Yishen capsule could reduce urinary protein level, it has little effect on blood glucose level in the rats injected with STZ.ConclusionsYishen capsule could attenuate the loss of podocalyxin in the glomerulus of rats injected with STZ.

【 授权许可】

CC BY   
© Fang et al.; licensee BioMed Central Ltd. 2013