Antibacterial constituents of three Cameroonian medicinal plants: Garcinia nobilis, Oricia suaveolens and Balsamocitrus camerunensis

BMC Complementary and Alternative Medicine

Research Article

Armelle T Mbaveng¹  Victor Kuete²  Augustin E Nkengfack³  Celine D Mbazoa³  Hugues Fouotsa⁴  Jacobus J Marion Meyer⁵  Namrita Lall⁵  Shaheen Farzana⁶  Choudhary M Iqbal⁶ 

[1] Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon;Department of Organic Chemistry, Higher Teachers Training College, University of Maroua, P.O.Box 46, Maroua, Cameroon;Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon;Department of Plant Science, Faculty of Agricultural and Biological Science, 0002, Pretoria, South Africa;Department of Organic Chemistry, Faculty of Science, Po.box 812, University of Yaoundé 1, Yaoundé, Cameroon;Department of Organic Chemistry, Faculty of Science, Po.box 812, University of Yaoundé 1, Yaoundé, Cameroon;H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, 75270, Karachi, Pakistan;Department of Plant Science, Faculty of Agricultural and Biological Science, 0002, Pretoria, South Africa;H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, 75270, Karachi, Pakistan;

关键词: Antimycobacterial;    Antibacterial;    Cameroon;    Compounds;    Garcinia nobilis;    Oricia suaveolens;    Balsamocitrus camerunensis;   

DOI  :  10.1186/1472-6882-13-81

 received in 2012-08-16, accepted in 2013-04-08,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundMultidrug resistance is a worrying cause of treatment failure in bacterial infections. The search of bioactive constituents from medicinal plants against multidrug resistant (MDR) bacteria has significantly evolved in the two last decades. In the present study, twenty-two compounds (three terpenoids, eleven phenolics and eight alkaloids) isolated from three Cameroonian medicinal plants, namely Garcinia nobilis, Oricia suaveolens and Balsamocitrus camerunensis, as well as the crude extracts were tested for their antibacterial activities against Mycobacterium tuberculosis and Gram-negative bacteria amongst which were MDR active efflux pumps expressing phenotypes.MethodsThe microplate alamar blue assay (MABA) and the broth microdilution methods were used to determine the minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) of the studied samples.ResultsThe results of the MIC determinations indicate that, the best crude extract was that from G. nobilis (GNB), its inhibitory effects being noted against 12 of the 14 tested bacteria. The extract of GNB also exhibited better anti-tuberculosis (MIC of 128 μg/ml M. tuberculosis against ATCC 27294 strain) and antibacterial (MIC of 64 μg/ml against Escherichia coli ATCC10536) activities compared to the extracts of O. suaveolens and B. camerunensis. Interestingly, 4-prenyl-2-(3,7-dimethyl-2,6-octadienyl)-1,3,5,8-tetrahydroxyxanthone (2), isolated from the most active extract GNB, also showed the best activity amongst compounds, inhibiting the growth of all the fourteen tested microorganisms. The lowest MIC value obtained with compound 2 was 8 μg/ml against M. tuberculosis ATCC 27294 and M. tuberculosis clinical MTCS2 strains. Other compounds showed selective activities with 11 of the 14 tested bacteria being sensitive to the xanthone, morusignin I (5) and the alkaloid, kokusaginine (13).ConclusionsThe results of the present investigation provide evidence that the crude extract from G. nobilis, O. suaveolens and B. camerunensis as well as some of their compounds, and mostly compound 2 (isolated from G. nobilis,) could be considered as interesting natural antibacterial products.

【 授权许可】

Unknown   
© Fouotsa et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.