Rapid detection of carbapenemase-producing Klebsiella pneumoniae strains derived from blood cultures by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)

BMC Microbiology

Researc Article

Silvia Angeletti¹  Camilla Dolfa²  Christina Sakarikou²  Cartesio Favalli³  Marco Ciotti⁴ 

[1] Clinical Pathology and Microbiology Laboratory, University Hospital Campus Bio-Medico, Via Alvaro del Portillo 200, 00128, Rome, Italy;Department of Experimental Medicine and Surgery, “Tor Vergata” University of Rome, Via Montpellier 1, 00133, Rome, Italy;Department of Experimental Medicine and Surgery, “Tor Vergata” University of Rome, Via Montpellier 1, 00133, Rome, Italy;Laboratory of Clinical Microbiology and Virology, Polyclinic “Tor Vergata” Foundation, V.le Oxford 81, 00133, Rome, Italy;Laboratory of Clinical Microbiology and Virology, Polyclinic “Tor Vergata” Foundation, V.le Oxford 81, 00133, Rome, Italy;

关键词: Carbapenemases;    Detection;    Klebsiella pneumoniae;    Blood culture;    MALDI-TOF MS;   

DOI  :  10.1186/s12866-017-0952-3

 received in 2016-10-09, accepted in 2017-02-09,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundCarbapenemase-producing Enterobacteriaceae (CPE), particularly carbapenemase-producing Klebsiella pneumoniae isolates, are important causative agents of nosocomial infections associated with significant mortality rates mostly in critical wards. The rapid detection and typing of these strains is critical either for surveillance purposes and to prevent outbreaks and optimize antibiotic therapy. In this study, the MALDI-TOF MS method was used to detect rapidly these isolates from blood cultures (BCs) and to obtain proteomic profiles enable to discriminate between carbapenemase-producing and non-carbapenemase-producing strains.ResultsFifty-five K. pneumoniae strains were tested. Identification and carbapenemase-production detection assay using Ertapenem were performed both from bacterial pellets extracted directly from BCs flasks and from subcultures of these strains. For all isolates, a complete antimicrobial susceptibility testing and a genotypic characterization were performed.We found 100% agreement between the carbapenemase-producing profile generated by MALDI TOF MS and that obtained using conventional methods. The assay detected and discriminated different carbapenemase-producing K. pneumoniae isolates within 30 min to 3 h after incubation with Ertapenem.ConclusionsMALDI-TOF MS is a promising, rapid and economical method for the detection of carbapenemase-producing K. pneumoniae strains that could be successfully introduced into the routine diagnostic workflow of clinical microbiology laboratories.

【 授权许可】

CC BY   
© The Author(s). 2017