Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype

BMC Infectious Diseases

Research Article

Daniel Gomes de Alvarenga¹  Elaine Speziali de Faria²  Sirlei Garcia Marques³  Vera Lúcia Santos⁴  Marcus Vinicíus Dias-Souza⁴  Jaqueline Gontijo de Sousa⁵  Kênia Valéria dos Santos⁶  Andrea de Souza Monteiro⁷  Elaine Alves de Oliveira Assunção⁸  Rodrigo Assunção de Holanda⁹  Maria Aparecida de Resende Stoianoff⁹  Lourimar Viana Nascimento F. de Sousa^1,10  Simone Gonçalves dos Santos^1,11  Juan Moises Zonis^1,12 

[1] Alvarenga Laboratory, Governador Valadares, MG, Brazil;Biomarkers Laboratory Diagnostics and Monitoring, Research Center René Rachou (CPqRR) - FIOCRUZ/MG, Belo Horizonte, MG, Brazil;Hospital of Federal University of Maranhão, São Luis, MA, Brazil;Laboratory of Applied Microbiology, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil;Laboratory of Basic and Applied Virology, Department of Microbiology, Institute of Biological Sciences, Federal University Minas Gerais, Belo Horizonte, MG, Brazil;Laboratory of General Bacteriology and Clinical, Department of Pathology, Federal University of Espirito Santo, Health Science Center, Vitória, ES, Brazil;Laboratory of Microbiology, CEUMA University, São Luis, MA, Brazil;Laboratory of Microbiology, Vale do Rio Doce University, Governador Valadares, MG, Brazil;Laboratory of Mycology, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil;Laboratory of Mycology, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil;Laboratory of Microbiology, Vale do Rio Doce University, Governador Valadares, MG, Brazil;Laboratory of oral and anaerobic microbiology, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil;Oncology Core Experts, Governador Valadares, MG, Brazil;

关键词: Candida;    Orogastric cancer;    Virulence;    Antifungal;    Immune phenotype;   

DOI  :  10.1186/s12879-016-1431-4

 received in 2015-05-12, accepted in 2016-02-12,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundBecause of the inherent immunosuppression of cancer patients opportunistic infections by Candida spp, occur frequently. This study aimed to identify Candida species in the oral mucosa of 59 patients with orogastric cancer (OGC) and to analyze the immunological phenotype of these patients.MethodsThe yeasts were identified by MALDI-TOF mass spectrometry (MS). For all isolates, we performed phospholipases and proteinases assays, in vitro adherence to buccal epithelial cells (BEC), minimum inhibitory concentration of antifungal drugs and determined the cytokine profile by Cytometric Bead Array flow citometry assay.ResultsC. albicans was the most prevalent species in OGC patients (51.6 %) and control group (66.7 %). Candida spp. strains isolated from OGC patients exhibited better adherence to BEC (p = 0.05) than did the control group. Phospholipases production by Candida strains from OGC patients was lower (51.6 %) than in the control group (61.9 %). Proteinases were detected in 41.9 % and 4.8 % of the yeasts from OGC patients and control group, respectively. Significant differences were found in the serum of OGC patients compared to the control group for IL-2, IL-10, TNF-α, IFN-γ and IL-17.ConclusionsThe results of this work suggest increased virulence of yeasts isolated from OGC patients and, that this may interfere with the immune phenotype.

【 授权许可】

CC BY   
© de Sousa et al. 2016