Cytotoxic and antimicrobial activity of selected Cameroonian edible plants

BMC Complementary and Alternative Medicine

Research Article

Santosh Kumar Guru¹  Ajit Kumar Saxena¹  Inshad Ali Khan²  Akash Sharma²  Victor Kuete³  Jean Paul Dzoyem⁴  Constant Anatole Pieme⁵  Ram Anuj Vishwakarma⁶ 

[1] Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Jammu, India;Clinical Microbiology Division, Indian Institute of Integrative Medicine, Jammu, India;Department of Biochemistry; Faculty of Science, University of Dschang, Dschang, Cameroon;Department of Biochemistry; Faculty of Science, University of Dschang, Dschang, Cameroon;Cancer Pharmacology Division, Indian Institute of Integrative Medicine, Jammu, India;Clinical Microbiology Division, Indian Institute of Integrative Medicine, Jammu, India;Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, Yaounde, Cameroon;Medicinal Chemistry Division, Indian Institute of Integrative Medicine, Jammu, India;

关键词: Cytotoxic;    Antimicrobial;    Edible plants;    Cameroon;   

DOI  :  10.1186/1472-6882-13-78

 received in 2013-02-01, accepted in 2013-04-05,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundIn Cameroon, the use of edible plants is an integral part of dietarybehavior. However, evidence of the antimicrobial as well as the cytotoxiceffects of many of them has not been investigated. In the present study,aqueous and methanol extracts from barks, seeds, leaves and roots of threeCameroonian edible plants namely Garcina lucida, Fagara heitzii andHymenocardia lyrata were evaluated for their cytotoxic andantimicrobial activities.MethodsAntibacterial and antifungal activities were assessed by the brothmicro-dilution method meanwhile the cytotoxicity was performed usingsulphorhodamine B assay (SRB) against the human leukemia THP-1, the alveolarepithelial A549, prostate cancer PC-3, breast adenocarcinoma MCF-7 andcervical cancer HeLa cell lines.ResultsThe minimum inhibitory concentration (MIC) values of the seven testedextracts ranged from 62.5 μg/ml to 1000 μg/ml. Themethanol (MeOH) extract from the roots of H. lyrata showed thehighest antibacterial activity against Gram-positive bacteria S.aureus and S. epidermitis. The best antifungal activitywas obtained with the MeOH extract from the leaves of G. lucidaagainst C. tropicalis (MIC value of 62.5 μg/ml). Thein vitro antiproliferative activity revealed that, extract fromthe bark of F. heitzii and extract from H. lyrata rootshad significant cytotoxic activity on THP-1 (IC508.4 μg/ml) and PC-3 (IC50 9.5 μg/ml)respectively.ConclusionOur findings suggest that Cameroonian spices herein studied could bepotentially useful for the development of therapeutic agents againstbacterial infections as well as for prostate and leukemia cancer.

【 授权许可】

CC BY   
© Dzoyem et al.; licensee BioMed Central Ltd. 2013