Mechanisms of gastroprotection of methanol extract of Melastoma malabathricum leaves

BMC Complementary and Alternative Medicine

Research Article

Siti Syariah Mamat¹  Tavamani Balan¹  Norhafizah Mohtarrudin²  Zainul Amiruddin Zakaria³  Mohd Zaki Salleh⁴  Teh Lay Kek⁴ 

[1] Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Serdang, Selangor, Malaysia;Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Serdang, Selangor, Malaysia;Halal Product Research Institute, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia;Integrative Pharmacogenomics Institute (iPROMISE), Faculty of Pharmacy, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia;Integrative Pharmacogenomics Institute (iPROMISE), Faculty of Pharmacy, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia;

关键词: Melastoma malabathricum;    Melastomaceae;    Methanol extract;    Gastroprotective mechanisms;    Nitric oxide;    Sulfhydryl group;    Antioxidant;    Anti-inflammatory;   

DOI  :  10.1186/s12906-015-0638-z

 received in 2014-09-14, accepted in 2015-03-30,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundMelastoma malabathricum L. (Melastomaceae) is a small shrub with various medicinal uses. The present study was carried out to determine the gastroprotective mechanisms of methanol extract of M. malabathricum leaves (MEMM) in rats.MethodsThe extract's mechanisms of gastroprotection (50, 250, 500 mg/kg) were studied using the pylorus-ligation in rat model wherein volume, pH, free and total acidity of gastric juice, and gastric wall mucus content were determined. The involvement of endogenous nitric oxide (NO) and sulfhydryl (SH) compounds in the gastroprotective effect of MEMM were also measured. MEMM was subjected to the antioxidant, anti-inflammatory and phytochemical analysis and HPLC profiling.ResultsMEMM contained various phyto-constituents with quercitrin being identified as part of them. MEMM and quercitrin: i) significantly (p < 0.05) reduced the volume and acidity of gastric juice while increasing the pH and gastric wall mucus content.; ii) significantly (p < 0.05) increased the level of SOD, GTP and GTR while significantly (p < 0.05) reduced the level of CAT, MPO and TBARS activities.; iii) exerted gastroprotective activity when assessed using the ethanol-induced gastric ulcer assay, which was reversed by NG-nitro-l-arginine methyl esters (L-NAME; an inhibitor of NO synthase) and N-ethylmaleimide (NEM; a sulfhydryl (SH) blocker). MEMM inhibited the lipoxygenase (LOX) and xanthine oxidase (XO) activities with the highest affinity for the former while quercitrin showed high affinity for XO activity.ConclusionsMEMM exhibited a gastroprotective activity due partly to the presence of quercitrin, its antioxidant and anti-inflammatory activities, and via the modulation of NO and SH groups.

【 授权许可】

CC BY   
© Zakaria et al. 2015