Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats

BMC Complementary and Alternative Medicine

Research Article

Krystal Feredoline Jakius¹  Mohamad Fauzi Fahmi Kamarolzaman¹  Farhana Yahya¹  Muhammad Syahmi Shahril¹  Nur Diyana Mahmood¹  Siti Syariah Mamat¹  Zainul Amiruddin Zakaria¹  Deny Susanti²  Siew Mooi Ching³  Norhafizah Mohtarrudin⁴  Muhammad Taher⁵ 

[1] Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia;Department of Biomedical Science, Kulliyyah of Science, International Islamic University Malaysia, Jl Sultan Ahmad Shah, Bandar Indera Mahkota, 25200, Kuantan, Pahang, Malaysia;Department of Family Medicine, Faculty of Medicine and Health Sciences, 43400, UPM Serdang, Selangor, Malaysia;Department of Pathology, Faculty of Medicine& Health Sciences, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia;Department of Pharmaceutical Technology, Kulliyyah of Pharmacy, International Islamic University Malaysia, Jalan Istana, Bandar Indera Mahkota, 25200, Kuantan, Pahang, Malaysia;

关键词: Melastoma malabathricum;    Melastomaceae;    Methanol extract;    Hepatoprotective activity;    Antioxidant activity;    Flavonoids;   

DOI  :  10.1186/1472-6882-13-326

 received in 2013-05-03, accepted in 2013-11-18,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundMelastoma malabathricum L. (Melastomaceae) is a small shrub with various medicinal uses. The present study was carried out to determine the hepatoprotective activity of methanol extract of M. malabathricum leaves (MEMM) against the paracetamol-induced liver toxicity in rats model.MethodsThe respective chemicals and herbal solutions (10% DMSO, 200 mg/kg silymarin or MEMM (50, 250 and 500 mg/kg)) were administered orally to rats once everyday for 7 days followed by the hepatotoxicity assay. The blood samples and livers were collected and subjected to biochemical and microscopical analysis. Prior to the hepatoprotective study, MEMM was subjected to determination of the total phenolic content (TPC) and the antioxidant properties using several standard assays (e.g. 2, 2-diphenyl-1-picrylhydrazyl- and superoxide anion- radical scavenging assay, and oxygen radical absorbance capacity assay).ResultsMEMM exerted significant (p < 0.05) and high antioxidant activity in which high TPC was recorded; while in the hepatotoxicity study, the extract exhibited significant hepatoprotective effects against the paracetamol-induced hepatotoxic model. The results observed for serum liver enzymes (ALT, ALP and AST) as well as the microscopic observations and microscopic scoring supported the hepatoprotective potential of MEMM. The phytochemical and HPLC analysis of MEMM demonstrated the presence of flavonoids as its major constituents.ConclusionsThe MEMM-induced hepatoprotective activity could be allied partly to its antioxidant activity and the presence of flavonoids.

【 授权许可】

Unknown   
© Mamat et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.