The utilization of nutmeg seed (Myristica fragrans Houtt) extract as an antimicrobial on tempeh sausage

International Conference on Agriculture, Environment, and Food Security 2018

农业科学;生态环境科学

Panggabean, K.A.^1 ; Rusmarilin, H.^1 ; Suryanto, D.^2

Department of Food Science, Faculty of Agriculture, Universitas Sumatera, Utara, Medan, North Sumatera, Indonesia^1

Department of Biology, Faculty of Mathematics and Natural Science, Universitas Sumatera, Utara, Medan, North Sumatera, Indonesia^2

关键词: Antimicrobial compounds;    Dilution method;    Ethyl acetates;    Methanol extract;    Microbial cells;    Minimum inhibitor concentrations;    Pathogenic microbes;    Staphylococcus aureus;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/260/1/012087/pdf
DOI  :  10.1088/1755-1315/260/1/012087

来源: IOP

【 摘 要 】

Nutmeg seed is an herb containing antimicrobial compounds and can be applied as an antimicrobial on foodstuffs. This study was aimed to know antimicrobial compounds of nutmeg seed extracting in water, methanol, ethyl acetate and hexane. Assay on the extract to inhibit pathogenic microbes (Staphylococcus aureus, Escherichia coli and Bacillus cereus) was conducted using dilution method. Phytochemical test was conducted to know the extract compounds. Minimum Inhibitor Concentration (MIC) test was measured to know minimum concentration of the extract to be applied in tempeh sausage. The results showed that nutmeg seed extract contained alkaloids, flavonoids, steroids, saponins, tannins and phenolics. Methanol extract was shown to have higher inhibition to the tested microbes compared to the other extracts. Minimum Inhibitor concentration (MIC) occurred at concentrations of 0.1% and 0.25% of nutmeg seed extract on Staphylococcus aureus and Escherichia coli. Nutmeg seed extract added on the tempeh sausage was able to reduce total microbial cell up to 5 days of storage compared to that of the control, i.e. 561x104 CFU/g for control, 61x104 CFU/g for 0,1% nutmeg seed extract and 54x104 CFU/g for 0,25% nutmeg seed extract.