Synergistic effect between Lactobacillus plantarum AKK30 and Saccharomyces cerevisiae B18 and the probiotic properties of microencapsulated cultures

2nd International Conference on Natural Products and Bioresource Sciences - 2018

生物科学;生态环境科学

Istiqomah, L.^1 ; Damayanti, E.^1 ; Suryani, A.E.^1 ; Karimy, M.F.^1 ; Maknuna, D.^2 ; Lutfiani^3

Research Unit for Natural Products Technology, Indonesian Institute of Sciences (BPTBA-LIPI), Jl. Jogja-Wonosari Km. 31.5, Gading, Playen, Gunungkidul, D.I. Yogyakarta

55861, Indonesia^1

Faculty of Science and Technology, Maulana Malik Ibrahim State Islamic University, Malang Jalan Gajayana No. 50, Malang

65144, Indonesia^2

Faculty of Mathematics and Science, Lambung Mangkurat University, Jl. Ahmad Yani Km 36 Banjarbaru Selatan, Loktabat Selatan, Banjarbaru, Kalimantan Selatan

70714, Indonesia^3

关键词: Animal feed additives;    Dilution method;    Lactic acid bacteria;    Lactobacillus plantarum;    Macroscopic and microscopic;    Microbial interactions;    Spray-drying method;    Synergistic effect;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/251/1/012062/pdf
DOI  :  10.1088/1755-1315/251/1/012062

来源: IOP

【 摘 要 】

The objectives of this study were to observe the microbial interactions between lactic acid bacteria (LAB - Lactobacillus plantarum AKK30) and yeast (S. cerevisiae B18) whether their effect is antagonistic or synergistic and to determine the potential of microencapsulated culture as probiotics for animal feed additive. The cell morphology of isolates were screened by macroscopic and microscopic examination. The synergistic effect of LAB and yeast was studied by disk diffusion and dilution method. The microencapsulated culture of LAB and yeast was prepared by spray drying method. Probiotic properties of microencapsulated cultures were examined for its viability on low pH, gastric juice and bile salt in vitro. The result of morphology cell showed that L. plantarum AKK30 had bacilli shaped with average length of cell was 1.35 μm at 48 h of incubation, whereas S. cerevisiae B18 had small budding characteristics with average length of cell was 3.19 μm at 96 h of incubation. The synergistic assay showed that L. plantarum AKK30 and S. cerevisiae B18 were alive and grew in a commensal relationship with diameter of clearing zone less than 5 mm and the population between two microorganisms was only 1 cycle log difference. Viability of microencapsulated culture was 97.25% for L. plantarum AKK30 and 91.66% for S. cerevisiae B18. The microencapsulated culture of L. plantarum AKK30 and S. cerevisiae B18 could be applied as probiotic for animal with good viability on pH 2 (95.18 and 87.95% respectively) and pH 3 (88.63 and 96.84% respectively) at 45 min of incubation, viability on gastric juice (66.58 and 92.70% respectively) at 45 min of incubation, and viability on 0.3% of bile salt (99.11 and 94.09% respectively) at 120 min of incubation. Thus, it could be concluded that LAB and yeast had synergistic effect and potentially use as a probiotic.