Study of phytochemical, anti-microbial, anti-oxidant, and anti-cancer properties of Allium wallichii

BMC Complementary and Alternative Medicine

Research Article

Pratiksha Thapa¹  Jaya Bhandari¹  Bhupal Govinda Shrestha¹  BushraTaj Muhammad² 

[1] Department of Biotechnology, Kathmandu University, Kathmandu, Nepal;Panjwani Centre for Molecular Medicine and Drug Research, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan;

关键词: Allium wallichii;    FACS;    MTT assay;    Cancer cell lines;    Cytotoxicity;    Anticancer agent;   

DOI  :  10.1186/s12906-017-1622-6

 received in 2016-06-17, accepted in 2017-02-03,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThere is growing interest in the use of plants for the treatment and prevention of cancer. Medicinal plants are currently being evaluated as source of promising anticancer agents. In this paper, we have investigated the anticancer potential of plant Allium wallichii, a plant native to Nepal and growing at elevations of 2300–4800 m. This is the first study of its kind for the plant mentioned.MethodsThe dried plant was extracted in aqueous ethanol. Phytochemical screening, anti-microbial assay, anti-oxidant assay, cytotoxicity assay and the flow-cytometric analysis were done for analyzing different phytochemicals present, anti-microbial activity, anti-oxidant activity and anti-cancer properties of Allium wallichii.ResultsWe observed the presence of steroids, terpenoids, flavonoids, reducing sugars and glycosides in the plant extract and the plant showed moderate anti-microbial and anti-oxidant activity. The IC50 values of Allium wallichii in different cancer cell lines are 69.69 μg/ml for Prostate cancer (PC3) cell line, 55.29 μg/ml for Breast Cancer (MCF-7) cell line and 46.51 μg/ml for cervical cancer (HeLa) cell line as compared to Doxorubicin (0.85 μg/ml). The cell viability assay using FACS showed that the IC50value of Allium wallichii for Burkitt’s lymphoma (B-Lymphoma) cell line was 3.817 ± 1.99 mg/ml.ConclusionsAllium wallichii can be an important candidate to be used as an anticancer agent. Separation of pure compounds with bioassay guided extraction, spectrometric analysis and subsequent cytotoxicity assay of the pure bioactive compounds from Allium wallichii is highly recommended as the crude extract itself showed promising cytotoxicity.

【 授权许可】

CC BY   
© The Author(s). 2017