BMC Microbiology | |
Identification of an unusual Brucella strain (BO2) from a lung biopsy in a 52 year-old patient with chronic destructive pneumonia | |
Research Article | |
Amy V Jennison1  John Bates1  David R Lonsway2  Jay E Gee2  Rebekah V Tiller2  Alex R Hoffmaster2  Barun K De2  Scott C Bell3  Chris Coulter4  Sonali Gribble4  | |
[1] Clinical and Statewide Services Division, Queensland Health, 39 Kessels Road, 4108, Coopers Plains, Queensland, Australia;Division of Foodborne, Bacterial, and Mycotic Diseases and Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, 1600 Clifton Road, 30333, Atlanta, GA, USA;Infection Management Service and Microbiology, The Prince Charles Hospital, Rode Road Chermside, 4032, Queensland, Australia;Infection Management Service and Microbiology, The Prince Charles Hospital, Rode Road Chermside, 4032, Queensland, Australia;Pathology Queensland Central Laboratory, Royal Brisbane and Women's Hospital, Herston Road, 4029, Queensland, Australia; | |
关键词: Brucellosis; Human Brucellosis; Brucella Species; Brucella Strain; Brucella Genus; | |
DOI : 10.1186/1471-2180-10-23 | |
received in 2009-08-25, accepted in 2010-01-27, 发布年份 2010 | |
来源: Springer | |
【 摘 要 】
BackgroundBrucellosis is primarily a zoonotic disease caused by Brucella species. There are currently ten Brucella spp. including the recently identified novel B. inopinata sp. isolated from a wound associated with a breast implant infection. In this study we report on the identification of an unusual Brucella-like strain (BO2) isolated from a lung biopsy in a 52-year-old patient in Australia with a clinical history of chronic destructive pneumonia.ResultsStandard biochemical profiles confirmed that the unusual strain was a member of the Brucella genus and the full-length 16S rRNA gene sequence was 100% identical to the recently identified B. inopinata sp. nov. (type strain BO1T). Additional sequence analysis of the recA, omp2a and 2b genes; and multiple locus sequence analysis (MLSA) demonstrated that strain BO2 exhibited significant similarity to the B. inopinata sp. compared to any of the other Brucella or Ochrobactrum species. Genotyping based on multiple-locus variable-number tandem repeat analysis (MLVA) established that the BO2 and BO1Tstrains form a distinct phylogenetic cluster separate from the other Brucella spp.ConclusionBased on these molecular and microbiological characterizations, we propose that the BO2 strain is a novel lineage of the newly described B. inopinata species.
【 授权许可】
Unknown
© Tiller et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
【 预 览 】
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