MLVA genotyping of Chinese human Brucella melitensisbiovar 1, 2 and 3 isolates

BMC Microbiology

Research Article

Jingdiao Chen¹  Changwen Ke¹  Xiaoling Deng¹  Ruiping Yu²  Mengguang Fan²  Jingchuan Mi²  Dongri Piao³  Hongyan Zhao³  Buyun Cui³  Hai Jiang³  Guozhong Tian³ 

[1] Department of Bacterial Pathogens, Institute for Pathogenic Microorganisms, Guangdong Center for Disease Control and Prevention, 176 Newport Road West, 510300, Guangzhou, PR, China;Department of Brucellosis, Inner Mongolia Center of Endemic Disease Control and Research, 50 Erdos Street, 010031, Huhhot, PR, China;State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 155 Changbai Road, Changping, 102206, Beijing, PR, China;

关键词: Vaccine Strain;    Brucellosis;    Human Brucellosis;    Brucella Melitensis;    Brucella Strain;   

DOI  :  10.1186/1471-2180-11-256

 received in 2011-05-06, accepted in 2011-11-22,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundSince 1950, Brucella melitensis has been the predominant strain associated with human brucellosis in China. In this study we investigated the genotypic characteristics of B. melitensis isolates from China using a multiple-locus variable-number tandem-repeat analysis (MLVA) and evaluated the utility of MLVA with regards to epidemiological trace-back investigation.ResultsA total of 105 B. melitensis strains isolated from throughout China were divided into 69 MLVA types using MLVA-16. Nei's genetic diversity indices for the various loci ranged between 0.00 - 0.84. 12 out 16 loci were the low diversity with values < 0.2 and the most discriminatory markers were bruce16 and bruce30 with a diversity index of > 0.75 and containing 8 and 7 alleles, respectively. Many isolates were single-locus or double-locus variants of closely related B. melitensis isolates from different regions, including the north and south of China. Using panel 1, the majority of strains (84/105) were genotype 42 clustering to the 'East Mediterranean' B. melitensis group. Chinese B. melitensis are classified in limited number of closely related genotypes showing variation mainly at the panel 2B loci.ConclusionThe MLVA-16 assay can be useful to reveal the predominant genotypes and strain relatedness in endemic or non-endemic regions of brucellosis. However it is not suitable for biovar differentiation of B. melitensis. Genotype 42 is widely distributed throughout China during a long time. Bruce 16 and bruce 30 in panel 2B markers are most useful for typing Chinese isolates.

【 授权许可】

Unknown   
© Jiang et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.