The genes and enzymes of the carotenoid metabolic pathway in Vitis vinifera L.

BMC Genomics

Research Article

Claudio Moser¹  Riccardo Velasco¹  Philip R Young²  Dan Jacobson²  Justin G Lashbrooke²  Melané A Vivier²  Erik Alexandersson³ 

[1] Genomics and Biology of Fruit Crops Department, IASMA Research and Innovation Centre, Fondazione Edmund Mach Via E. Mach 1, 38010, San Michele all'Adige, TN, Italy;Institute for Wine Biotechnology, Department of Viticulture and Oenology, Stellenbosch University, 7602, Matieland, South Africa;Institute for Wine Biotechnology, Department of Viticulture and Oenology, Stellenbosch University, 7602, Matieland, South Africa;Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-230 53, Alnarp, Sweden;

关键词: Carotenoid;    Lycopene;    Zeaxanthin;    Violaxanthin;    Grape Berry;   

DOI  :  10.1186/1471-2164-13-243

 received in 2011-12-15, accepted in 2012-05-03,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundCarotenoids are a heterogeneous group of plant isoprenoids primarily involved in photosynthesis. In plants the cleavage of carotenoids leads to the formation of the phytohormones abscisic acid and strigolactone, and C13-norisoprenoids involved in the characteristic flavour and aroma compounds in flowers and fruits and are of specific importance in the varietal character of grapes and wine. This work extends the previous reports of carotenoid gene expression and photosynthetic pigment analysis by providing an up-to-date pathway analysis and an important framework for the analysis of carotenoid metabolic pathways in grapevine.ResultsComparative genomics was used to identify 42 genes putatively involved in carotenoid biosynthesis/catabolism in grapevine. The genes are distributed on 16 of the 19 chromosomes and have been localised to the physical map of the heterozygous ENTAV115 grapevine sequence. Nine of the genes occur as single copies whereas the rest of the carotenoid metabolic genes have more than one paralogue. The cDNA copies of eleven corresponding genes from Vitis vinifera L. cv. Pinotage were characterised, and four where shown to be functional. Microarrays provided expression profiles of 39 accessions in the metabolic pathway during three berry developmental stages in Sauvignon blanc, whereas an optimised HPLC analysis provided the concentrations of individual carotenoids. This provides evidence of the functioning of the lutein epoxide cycle and the respective genes in grapevine. Similarly, orthologues of genes leading to the formation of strigolactone involved in shoot branching inhibition were identified: CCD7, CCD8 and MAX1. Moreover, the isoforms typically have different expression patterns, confirming the complex regulation of the pathway. Of particular interest is the expression pattern of the three VvNCEDs: Our results support previous findings that VvNCED3 is likely the isoform linked to ABA content in berries.ConclusionsThe carotenoid metabolic pathway is well characterised, and the genes and enzymes have been studied in a number of plants. The study of the 42 carotenoid pathway genes of grapevine showed that they share a high degree of similarity with other eudicots. Expression and pigment profiling of developing berries provided insights into the most complete grapevine carotenoid pathway representation. This study represents an important reference study for further characterisation of carotenoid biosynthesis and catabolism in grapevine.

【 授权许可】

Unknown   
© Young et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.