Complete separation of the β,ε‐ and β,β‐carotenoid biosynthetic pathways by a unique mutation of the lycopene cyclase in the green alga, Scenedesmus obliquus

FEBS Letters

Bishop, Norman I.¹  Senger, Horst²  Urbig, Thomas² 

[1] Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA;Fachbereich Biologie/Botanik, Philipps-Universitat Marburg, Lahnberge, D-35039 Marburg, Germany

关键词: β;    β- and β;    ε-Carotenoid;    Chloroplast development;    Light-harvesting complex;    Lutein;    Photosynthetic apparatus;    Mutants of Scenedesmus;    Carotenoids Antheraxanthin;    (3S;    5R;    6S;    3′R)-5;    6-Epoxy-5;    6-dihydro-β-β-carotene-3;    3′diol;    Loroxanthin;    (3R;    3′R;    6′R)-β;    ε-Carotene-3;    19;    3′triol;    Lutein;    (3R;    3′R;    6′R)-β;    ε-Carotene-3;    3′diol;    Neoxanthin;    (3S;    5R;    6R;    3′8;    5′R;    6′8)-5′;    6′-Epoxy-6;    7-didehydro-5;    6;    5′;    6′-tetrahydro-β;    β-Carotene-3;    5;    3′ triol;    Violaxanthin;    (3R;    5R;    6S;    5′R;    6′S)-5;    6;    5′;    6′-Diepoxy-5;    6;    5′;    6′-tetradehydro-β;    β-Carotene-3-3′diol;    Zeaxanthin;    (3R;    3′R)-β;    β-carotene-3;    3′-diol;    Chl a;    chlorophyll a;    Chl b;    chlorophyll b;    EMS;    ethyl ester of methylsulfonic acid;    LHC;    light harvesting complex;    PCV;    packed cell volume;    PPC;    pigment-protein complex;    PS-I;    photosystem I;    PS- II;    photosystem II;    rpHPLC;    reversed phase high performance liquid chromatography;    WT;    normal wild-type of Scenedesmus;   

DOI  :  10.1016/0014-5793(95)00510-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mutant, C-2A′-34, lacks the β, ϵ-carotenoids, α-carotene, lutein and loroxanthin. When grown under heterotrophic or mixotropic conditions this strain develops significantly higher levels of β-carotene and violaxanthin than does the original developmental mutant of Scenedesmus, C-2A′. The decrease in chlorophyll a and chlorophyll b observed in C-2A′-34 is accompanied by the near absence of the LHC. The light intensity dependence of greening of this strain is comparable to that of C-2A′; the loss of the β,ϵ-carotenoids and modification of the pool of β,β-carotenoids neither prevent the proximal pigment-protein complexes of photosystems I and II from developing nor cause any short term photosensitivity. The increase in the β,β-carotenoids in C-2A′-34 apparently compensates for the loss of the β,ε-carotenoids required in formation of the proximal and distal antennae systems but not in the LHCs.

【 授权许可】

Unknown