Cynomorium songaricum extract enhances novel object recognition, cell proliferation and neuroblast differentiation in the mice via improving hippocampal environment

BMC Complementary and Alternative Medicine

Research Article

In Koo Hwang¹  Dae Young Yoo¹  Yeo Sung Yoon¹  Sung Min Nam¹  Jong Whi Kim¹  Woosuk Kim¹  Hyo Young Jung¹  Jung Hoon Choi²  Moo-Ho Won³  Ki-Yeon Yoo⁴ 

[1] Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, 151-742, Seoul, South Korea;Department of Anatomy, College of Veterinary Medicine, Kangwon National University, 200-701, Chuncheon, South Korea;Department of Neurobiology, School of Medicine, Kangwon National University, 200-701, Chuncheon, South Korea;Department of Oral Anatomy, College of Dentistry, Research Institute of Oral Sciences, Gangneung-Wonju National University, 210-702, Gangneung, South Korea;

关键词: Cynomorium songaricum;    Hippocampus;    5-bromodeoxyuridine;    Polysialylated neural cell adhesion molecule;    Corticosterone;   

DOI  :  10.1186/1472-6882-14-5

 received in 2013-09-09, accepted in 2013-12-31,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundCynomorium songaricum Rupr. (CS) has been used as a medicine to treat many diseases as well as to alleviate age-related issues, such as memory impairment, dementia, and stress. In this study, we assessed the effects of Cynomorium songaricum extract (CSE) on the novel object recognition, cell proliferation and neuroblast differentiation in the dentate gyrus of mice by using 5-bromodeoxyuridine (BrdU) and polysialylated neural cell adhesion molecule (PSA-NCAM). We also measured serum corticosterone levels to assess its correlation with neurogenesis and stress.MethodsMale C57BL/6 J mice were divided into 3 groups: vehicle-treated, 40 mg/kg CSE-treated, and 100 mg/kg CSE-treated. The vehicle and CSE were given to mice once a day for 3 weeks. BrdU was injected twice a day for 3 days to label newly generated cells.ResultsAdministration of CSE significantly increased the preferential exploration of new objects in these mice. In addition, administration of CSE decreased serum levels of corticosterone. BrdU-positive cells as well as brain-derived neurotrophic factor (BDNF) mRNA expression in the dentate gyrus were higher in the CSE-treated groups than in the vehicle-treated group. PSA-NCAM-positive neuroblasts and their well-developed tertiary dendrites were also significantly increased by the treatment of CSE. These effects were prominent at the higher dosage than at the lower dosage.ConclusionThese results suggest that administration of CSE increases cell proliferation and neuroblast differentiation in the dentate gyrus of mice by reducing serum corticosterone levels and increasing BDNF levels in this area.

【 授权许可】

Unknown   
© Yoo et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.