The correlation between pretreatment cytokine expression patterns in peripheral blood mononuclear cells with chronic hepatitis c outcome

BMC Infectious Diseases

Research Article

Sylwia Osowska¹  Joanna Jabłońska²  Kamila Caraballo Cortes³  Karol Perlejewski³  Iwona Bukowska-Ośko³  Marek Radkowski³  Agnieszka Pawełczyk³  Tomasz Laskus³  Małgorzata Zalewska⁴  Małgorzata Inglot⁴  Tomasz Pawłowski⁵  Piotr Ząbek⁶ 

[1] Department of General Surgery and Clinical Nutrition, Medical University of Warsaw, Warsaw, Poland;Department of Hepatology and Acquired Immunodeficiences, Medical University of Warsaw, Warsaw, Poland;Department of Immunopathology of Infectious Diseases, Medical University of Warsaw, Warsaw, Poland;Department of Infectious Diseases, Hepatology and Acquired Immune Deficiences, Wrocław Medical University, Wrocław, Poland;Division of Psychotherapy and Psychosomatic Medicine, Wrocław Medical University, Wrocław, Poland;Municipal Hospital of Infectious Diseases, Warsaw, Poland;

关键词: HCV;    Antiviral treatment;    PBMC;    Cytokines;    Gene expression;   

DOI  :  10.1186/s12879-015-1305-1

 received in 2014-06-23, accepted in 2015-12-01,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroudCytokine response against hepatitis C virus (HCV) is likely to determine the natural course of infection as well as the outcome of antiviral treatment. However, the role of particular cytokines remains unclear. The current study analyzed activation of cytokine response in chronic hepatitis C patients undergoing standard antiviral treatment.MethodsTwenty-two patients were treated with pegylated interferon and ribavirin. Twenty-six different cytokine transcripts were measured quantitatively in peripheral blood mononuclear cells (PBMC) before and after therapy and correlated with therapy outcome as well as with clinical and liver histological data.ResultsWe found that patients who achieved sustained virological response (SVR) showed higher pretreatment cytokine response when compared to subjects in whom therapy was unsuccessful. The differentially expressed factors included IL-8, IL-16, TNF-α, GM-CSF, MCP-2, TGF-β, and IP-10. Serum ALT activity and/or histological grading also positively correlated with the expression of IL-1α, IL-4, IL-6, IL-10, IL-12, IL-15, GM-CSF, M-CSF, MCP-2 and TGF-β.ConclusionPretreatment activation of the immune system, as reflected by cytokines transcripts upregulation, positively correlates with treatment outcome and closely reflects liver inflammatory activity.

【 授权许可】

CC BY   
© Jabłońska et al. 2015