BMC Microbiology | |
Cell surface sialylation affects binding of enterovirus 71 to rhabdomyosarcoma and neuroblastoma cells | |
Research Article | |
Yueh-Tung Liu1  Hsin-Yueh Chang2  Ya-Fang Wang2  Chuan-Fa Chang3  Sheng-Wen Huang3  Jen-Ren Wang3  Pei-Yi Su4  Chun-Keung Yu5  | |
[1] Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, No. 1, University Road, 70101, Tainan, Taiwan;Blood Bank, Kaohsiung Veterans General Hospital, No. 386, Ta-Chung 1st Road, 81362, Kaohsiung, Taiwan;Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, No. 1, University Road, 70101, Tainan, Taiwan;Center of Infectious Disease and Signaling Research, Medical College, National Cheng Kung University, No. 1, University Road, 70101, Tainan, Taiwan;Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, No. 1, University Road, 70101, Tainan, Taiwan;Institute of Basic Medical Sciences, National Cheng Kung University, No. 1, University Road, 70101, Tainan, Taiwan;Center of Infectious Disease and Signaling Research, Medical College, National Cheng Kung University, No. 1, University Road, 70101, Tainan, Taiwan;Institute of Basic Medical Sciences, National Cheng Kung University, No. 1, University Road, 70101, Tainan, Taiwan;Center of Infectious Disease and Signaling Research, Medical College, National Cheng Kung University, No. 1, University Road, 70101, Tainan, Taiwan;Institute of Basic Medical Sciences, National Cheng Kung University, No. 1, University Road, 70101, Tainan, Taiwan;Center of Infectious Disease and Signaling Research, Medical College, National Cheng Kung University, No. 1, University Road, 70101, Tainan, Taiwan;Department of Microbiology and Immunology, National Cheng Kung University, No. 1, University Road, 70101, Tainan, Taiwan;National Applied Research Laboratories, National Laboratory Animal Center, No. 128 Academia Road Section 2, 11529, Nan-Kang, Taipei, Taiwan; | |
关键词: Enterovirus 71; Sialic acid; RD; SK-N-SH; Lectin affinity chromatography; | |
DOI : 10.1186/1471-2180-12-162 | |
received in 2012-05-07, accepted in 2012-07-24, 发布年份 2012 | |
来源: Springer | |
【 摘 要 】
BackgroundEnterovirus 71 (EV71) is a major causative agent of hand-foot-and-mouth disease (HFMD), and infection of EV71 to central nerve system (CNS) may result in a high mortality in children less than 2 years old. Although there are two highly glycosylated membrane proteins, SCARB2 and PSGL-1, which have been identified as the cellular and functional receptors of EV71, the role of glycosylation in EV71 infection is still unclear.ResultsWe demonstrated that the attachment of EV71 to RD and SK-N-SH cells was diminished after the removal of cell surface sialic acids by neuraminidase. Sialic acid specific lectins, Maackia amurensis (MAA) and Sambucus Nigra (SNA), could compete with EV71 and restrained the binding of EV71 significantly. Preincubation of RD cells with fetuin also reduced the binding of EV71. In addition, we found that SCARB2 was a sialylated glycoprotein and interaction between SCARB2 and EV71 was retarded after desialylation.ConclusionsIn this study, we demonstrated that cell surface sialic acids assist in the attachment of EV71 to host cells. Cell surface sialylation should be a key regulator that facilitates the binding and infection of EV71 to RD and SK-N-SH cells.
【 授权许可】
Unknown
© Su et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202311094898060ZK.pdf | 2138KB | download |
【 参考文献 】
- [1]
- [2]
- [3]
- [4]
- [5]
- [6]
- [7]
- [8]
- [9]
- [10]
- [11]
- [12]
- [13]
- [14]
- [15]
- [16]
- [17]
- [18]
- [19]
- [20]
- [21]
- [22]
- [23]
- [24]
- [25]
- [26]
- [27]
- [28]
- [29]
- [30]
- [31]
- [32]
- [33]
- [34]
- [35]
- [36]
- [37]
- [38]
- [39]
- [40]
- [41]
- [42]
- [43]
- [44]
- [45]
- [46]