Cell surface sialylation affects binding of enterovirus 71 to rhabdomyosarcoma and neuroblastoma cells

BMC Microbiology

Chuan-Fa Chang¹  Jen-Ren Wang¹  Chun-Keung Yu²  Ya-Fang Wang¹  Sheng-Wen Huang¹  Hsin-Yueh Chang¹  Yueh-Tung Liu³  Pei-Yi Su¹ 

[1] Center of Infectious Disease and Signaling Research, Medical College, National Cheng Kung University, No. 1, University Road, Tainan 70101, Taiwan;National Applied Research Laboratories, National Laboratory Animal Center, No. 128 Academia Road Section 2, Nan-Kang, Taipei 11529, Taiwan;Blood Bank, Kaohsiung Veterans General Hospital, No. 386, Ta-Chung 1st Road, Kaohsiung 81362, Taiwan

关键词: Lectin affinity chromatography;    SK-N-SH;    RD;    Sialic acid;    Enterovirus 71;   

Others  :  1221802
DOI  :  10.1186/1471-2180-12-162

 received in 2012-05-07, accepted in 2012-07-24,  发布年份 2012

【 摘 要 】

Background

Enterovirus 71 (EV71) is a major causative agent of hand-foot-and-mouth disease (HFMD), and infection of EV71 to central nerve system (CNS) may result in a high mortality in children less than 2 years old. Although there are two highly glycosylated membrane proteins, SCARB2 and PSGL-1, which have been identified as the cellular and functional receptors of EV71, the role of glycosylation in EV71 infection is still unclear.

Results

We demonstrated that the attachment of EV71 to RD and SK-N-SH cells was diminished after the removal of cell surface sialic acids by neuraminidase. Sialic acid specific lectins, Maackia amurensis (MAA) and Sambucus Nigra (SNA), could compete with EV71 and restrained the binding of EV71 significantly. Preincubation of RD cells with fetuin also reduced the binding of EV71. In addition, we found that SCARB2 was a sialylated glycoprotein and interaction between SCARB2 and EV71 was retarded after desialylation.

Conclusions

In this study, we demonstrated that cell surface sialic acids assist in the attachment of EV71 to host cells. Cell surface sialylation should be a key regulator that facilitates the binding and infection of EV71 to RD and SK-N-SH cells.

【 授权许可】

   
2012 Su et al.; licensee BioMed Central Ltd.

【 图 表 】

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