Establishment and metabolic analysis of a model microbial community for understanding trophic and electron accepting interactions of subsurface anaerobic environments

BMC Microbiology

Research Article

Jennifer J Mosher¹  Christopher W Schadt¹  Lance D Miller¹  Zamin K Yang¹  Mircea Podar¹  Anthony V Palumbo¹  Tommy J Phelps¹  Martin Keller¹  Amudhan Venkateswaran¹ 

[1] Biosciences and Environmental Sciences Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA;Virtual Institute for Microbial Stress and Survival, LBNL One Cyclotron Road MS 977-152, 94720, Berkeley, CA, USA;

关键词: Cellobiose;    Fumarate;    Hydrogen Sulfide;    Species Community;    Thioctic Acid;   

DOI  :  10.1186/1471-2180-10-149

 received in 2009-12-09, accepted in 2010-05-24,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundCommunities of microorganisms control the rates of key biogeochemical cycles, and are important for biotechnology, bioremediation, and industrial microbiological processes. For this reason, we constructed a model microbial community comprised of three species dependent on trophic interactions. The three species microbial community was comprised of Clostridium cellulolyticum, Desulfovibrio vulgaris Hildenborough, and Geobacter sulfurreducens and was grown under continuous culture conditions. Cellobiose served as the carbon and energy source for C. cellulolyticum, whereas D. vulgaris and G. sulfurreducens derived carbon and energy from the metabolic products of cellobiose fermentation and were provided with sulfate and fumarate respectively as electron acceptors.ResultsqPCR monitoring of the culture revealed C. cellulolyticum to be dominant as expected and confirmed the presence of D. vulgaris and G. sulfurreducens. Proposed metabolic modeling of carbon and electron flow of the three-species community indicated that the growth of C. cellulolyticum and D. vulgaris were electron donor limited whereas G. sulfurreducens was electron acceptor limited.ConclusionsThe results demonstrate that C. cellulolyticum, D. vulgaris, and G. sulfurreducens can be grown in coculture in a continuous culture system in which D. vulgaris and G. sulfurreducens are dependent upon the metabolic byproducts of C. cellulolyticum for nutrients. This represents a step towards developing a tractable model ecosystem comprised of members representing the functional groups of a trophic network.

【 授权许可】

Unknown   
© Miller et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.