Comparative proteomics of Rhizopus delemar ATCC 20344 unravels the role of amino acid catabolism in fumarate accumulation

PeerJ

article

Dorett I. Odoni¹  Juan A. Tamayo-Ramos¹  Jasper Sloothaak¹  Ruben G.A. van Heck¹  Vitor A.P. Martins dos Santos¹  Leo H. de Graaff¹  Maria Suarez-Diez¹  Peter J. Schaap¹ 

[1] Laboratory of Systems and Synthetic Biology, Wageningen University & Research;LifeGlimmer GmBH

关键词: Rhizopus delemar;    Fumarate;    Proteomics;    Transcriptomics;    Amino acid metabolism;    Nitrogen metabolism;   

DOI  :  10.7717/peerj.3133

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The filamentous fungus Rhizopus delemar naturally accumulates relatively high amounts of fumarate. Although the culture conditions that increase fumarate yields are well established, the network underlying the accumulation of fumarate is not yet fully understood. We set out to increase the knowledge about fumarate accumulation in R. delemar. To this end, we combined a transcriptomics and proteomics approach to identify key metabolic pathways involved in fumarate production in R. delemar, and propose that a substantial part of the fumarate accumulated in R. delemar during nitrogen starvation results from the urea cycle due to amino acid catabolism.

【 授权许可】

CC BY