The tricarboxylic acid cycle in Shewanella oneidensis is independent of Fur and RyhB control

BMC Microbiology

Research Article

Andrea B Parsons¹  Sheng Feng²  Yunfeng Yang³  Jizhong Zhou⁴  Lee Ann McCue⁵ 

[1] Biosciences Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA;Department of Biostatistics and Bioinformatics, Duke University, 27710, Durham, NC, USA;Department of Environmental Science and Engineering, Tsinghua University, 100084, Beijing, China;Biosciences Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA;Department of Environmental Science and Engineering, Tsinghua University, 100084, Beijing, China;Biosciences Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA;Institute for Environmental Genomics and Department of Botany and Microbiology, University of Oklahoma, 73019, Norman, OK, USA;Pacific Northwest National Laboratory, 99352, Richland, WA, USA;

关键词: Succinate;    Fumarate;    cAMP Receptor Protein;    Shewanella Species;    Ferric Uptake Regulator;   

DOI  :  10.1186/1471-2180-10-264

 received in 2010-04-23, accepted in 2010-10-16,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundIt is well established in E. coli and Vibrio cholerae that strains harboring mutations in the ferric uptake regulator gene (fur) are unable to utilize tricarboxylic acid (TCA) compounds, due to the down-regulation of key TCA cycle enzymes, such as AcnA and SdhABCD. This down-regulation is mediated by a Fur-regulated small regulatory RNA named RyhB. It is unclear in the γ-proteobacterium S. oneidensis whether TCA is also regulated by Fur and RyhB.ResultsIn the present study, we showed that a fur deletion mutant of S. oneidensis could utilize TCA compounds. Consistently, expression of the TCA cycle genes acnA and sdhA was not down-regulated in the mutant. To explore this observation further, we identified a ryhB gene in Shewanella species and experimentally demonstrated the gene expression. Further experiments suggested that RyhB was up-regulated in fur mutant, but that AcnA and SdhA were not controlled by RyhB.ConclusionsThese cumulative results delineate an important difference of the Fur-RyhB regulatory cycle between S. oneidensis and other γ-proteobacteria. This work represents a step forward for understanding the unique regulation in S. oneidensis.

【 授权许可】

CC BY   
© Yang et al; licensee BioMed Central Ltd. 2010