Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in Yersinia pestis

BMC Microbiology

Research Article

Xinxiang Huang¹  Yiquan Zhang²  Dongsheng Zhou²  Ruifu Yang²  Yafang Tan²  Zhaobiao Guo²  He Gao²  Yanping Han²  Lin Yang³  Xia Liu³ 

[1] Department of Biochemistry and Molecular Biology, Jiangsu University School of Medical Technology, 212013, Zhenjiang, Jiangsu, PR China;State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 100071, Beijing, PR China;State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 100071, Beijing, PR China;Department of Biochemistry and Molecular Biology, Jiangsu University School of Medical Technology, 212013, Zhenjiang, Jiangsu, PR China;

关键词: cAMP Receptor Protein;    Medium Osmolarity;    Primer Extension Assay;    Primer Extension Product;    Cellular cAMP Level;   

DOI  :  10.1186/1471-2180-11-40

 received in 2010-06-11, accepted in 2011-02-23,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe cAMP receptor protein (CRP) is a global bacterial regulator that controls many target genes. The CRP-cAMP complex regulates the ompR-envZ operon in E. coli directly, involving both positive and negative regulations of multiple target promoters; further, it controls the production of porins indirectly through its direct action on ompR-envZ. Auto-regulation of CRP has also been established in E. coli. However, the regulation of porin genes and its own gene by CRP remains unclear in Y. pestis.ResultsY. pestis employs a distinct mechanism indicating that CRP has no regulatory effect on the ompR-envZ operon; however, it stimulates ompC and ompF directly, while repressing ompX. No transcriptional regulatory association between CRP and its own gene can be detected in Y. pestis, which is also in contrast to the fact that CRP acts as both repressor and activator for its own gene in E. coli. It is likely that Y. pestis OmpR and CRP respectively sense different signals (medium osmolarity, and cellular cAMP levels) to regulate porin genes independently.ConclusionAlthough the CRP of Y. pestis shows a very high homology to that of E. coli, and the consensus DNA sequence recognized by CRP is shared by the two bacteria, the Y. pestis CRP can recognize the promoters of ompC, F, and X directly rather than that of its own gene, which is different from the relevant regulatory circuit of E. coli. Data presented here indicate a remarkable remodeling of the CRP-mediated regulation of porin genes and of its own one between these two bacteria.

【 授权许可】

Unknown   
© Gao et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.