FGF2-induced effects on transcriptome associated with regeneration competence in adult human fibroblasts

BMC Genomics

Research Article

Tanja Dominko¹  Olga Kashpur¹  Elizabeth F Ryder¹  Sakthikumar Ambady²  David LaPointe³ 

[1] Department of Biology and Biotechnology, Worcester Polytechnic Institute, 100 Institute Road, 01609, Worcester, MA, USA;Department of Biomedical Engineering, Worcester Polytechnic Institute, 100 Institute Road, 01609, Worcester, MA, USA;Department of Cell and Developmental Biology, University of Massachusetts Medical School, 55 Lake Avenue North, 01655, Worcester, MA, USA;

关键词: Transcriptome;    Human fibroblasts;    Fibroblast growth factor (FGF2);    Wound healing;    Regeneration;   

DOI  :  10.1186/1471-2164-14-656

 received in 2013-03-20, accepted in 2013-09-24,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundAdult human fibroblasts grown in low oxygen and with FGF2 supplementation have the capacity to tip the healing outcome of skeletal muscle injury – by favoring regeneration response in vivo over scar formation. Here, we compare the transcriptomes of control adult human dermal fibroblasts and induced regeneration-competent (iRC) fibroblasts to identify transcriptional changes that may be related to their regeneration competence.ResultsWe identified a unique gene-expression profile that characterizes FGF2-induced iRC fibroblast phenotype. Significantly differentially expressed genes due to FGF2 treatment were identified and analyzed to determine overrepresented Gene Ontology terms. Genes belonging to extracellular matrix components, adhesion molecules, matrix remodelling, cytoskeleton, and cytokines were determined to be affected by FGF2 treatment.ConclusionsTranscriptome analysis comparing control adult human fibroblasts with FGF2-treated fibroblasts identified functional groups of genes that reflect transcriptional changes potentially contributing to their regeneration competence. This comparative transcriptome analysis should contribute new insights into genes that characterize cells with greater regenerative potential.

【 授权许可】

Unknown   
© Kashpur et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.