Identification and use of the sugarcane bacilliform virus enhancer in transgenic maize

BMC Plant Biology

Research Article

John P Davies¹  Xing L Liu¹  Vaka Reddy²  Douglas Ry Wagner³  Delkin O Gonzalez⁴  James Connell⁴  Mark Thompson⁴  Avutu S Reddy⁴  William Michael Ainley⁴  Lakshmi Sastry-Dent⁴  Zehui Cao⁴ 

[1] Dow AgroSciences, 16160 SW Upper Boones Ferry Rd, 97224, Portland, OR, USA;Dow AgroSciences, 16160 SW Upper Boones Ferry Rd, 97224, Portland, OR, USA;Current address: GEVO, Inc., 345 Inverness Dr S C-310, 80112, Englewood, CO, USA;Dow AgroSciences, 16160 SW Upper Boones Ferry Rd, 97224, Portland, OR, USA;Dow AgroSciences, 9330 Zionsville Rd, 46268, Indianapolis, IN, USA;Current address: Agrinos, Inc, 279 Cousteau Place, 95618, Davis, CA, USA;Dow AgroSciences, 9330 Zionsville Rd, 46268, Indianapolis, IN, USA;

关键词: Promoter;    Enhancer;    Transcription;    Transgenic plant;    Transient assay;   

DOI  :  10.1186/s12870-014-0359-3

 received in 2014-08-22, accepted in 2014-11-27,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundTranscriptional enhancers are able to increase transcription from heterologous promoters when placed upstream, downstream and in either orientation, relative to the promoter. Transcriptional enhancers have been used to enhance expression of specific promoters in transgenic plants and in activation tagging studies to help elucidate gene function.ResultsA transcriptional enhancer from the Sugarcane Bacilliform Virus - Ireng Maleng isolate (SCBV-IM) that can cause increased transcription when integrated into the the genome near maize genes has been identified. In transgenic maize, the SCBV-IM promoter was shown to be comparable in strength to the maize ubiquitin 1 promoter in young leaf and root tissues. The promoter was dissected to identify sequences that confer high activity in transient assays. Enhancer sequences were identified and shown to increase the activity of a heterologous truncated promoter. These enhancer sequences were shown to be more active when arrayed in 4 copy arrays than in 1 or 2 copy arrays. When the enhancer array was transformed into maize plants it caused an increase in accumulation of transcripts of genes near the site of integration in the genome.ConclusionsThe SCBV-IM enhancer can activate transcription upstream or downstream of genes and in either orientation. It may be a useful tool to activate enhance from specific promoters or in activation tagging.

【 授权许可】

CC BY   
© Davies et al.; licensee BioMed Central. 2014