Multiple conserved regulatory domains promote Fezf2 expression in the developing cerebral cortex

Neural Development

Bin Chen¹  John L R Rubenstein²  Axel Visel⁴  Robin Roque¹  Chao Guo¹  Sol Katzman³  William L McKenna¹  Kathryn A Larkin¹  Matthew J Eckler¹ 

[1] Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, CA, USA;Nina Ireland Laboratory of Developmental Neurobiology, Department of Psychiatry, University of California, San Francisco, CA, USA;Center for Biomolecular Science and Engineering, University of California, Santa Cruz, CA, USA;U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA, USA

关键词: Transcription;    Gene regulation;    Cerebral cortex;    Promoter;    Enhancer;    Fezf2;   

Others  :  803180
DOI  :  10.1186/1749-8104-9-6

 received in 2013-12-03, accepted in 2014-02-19,  发布年份 2014

【 摘 要 】

Background

The genetic programs required for development of the cerebral cortex are under intense investigation. However, non-coding DNA elements that control the expression of developmentally important genes remain poorly defined. Here we investigate the regulation of Fezf2, a transcription factor that is necessary for the generation of deep-layer cortical projection neurons.

Results

Using a combination of chromatin immunoprecipitation followed by high throughput sequencing (ChIP-seq) we mapped the binding of four deep-layer-enriched transcription factors previously shown to be important for cortical development. Building upon this we characterized the activity of three regulatory regions around the Fezf2 locus at multiple stages throughout corticogenesis. We identified a promoter that was sufficient for expression in the cerebral cortex, and enhancers that drove reporter gene expression in distinct forebrain domains, including progenitor cells and cortical projection neurons.

Conclusions

These results provide insight into the regulatory logic controlling Fezf2 expression and further the understanding of how multiple non-coding regulatory domains can collaborate to control gene expression in vivo.

【 授权许可】

   
2014 Eckler et al.; licensee BioMed Central Ltd.

【 图 表 】

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